Browsing by Author "Kogan, Marcelo J."
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- ItemExtracellular vesicles through the blood–brain barrier: a review(2022) Ramos Zaldívar, Héctor M.; Polakovicova, Iva; Salas-Huenuleo, Edison; Corvalán R., Alejandro; Kogan, Marcelo J.; Yefi Rubio, Claudia Pamela; Andía Kohnenkampf, Marcelo EdgardoExtracellular vesicles (EVs) are particles naturally released from cells that are delimited by a lipid bilayer and are unable to replicate. How the EVs cross the Blood–Brain barrier (BBB) in a bidirectional manner between the bloodstream and brain parenchyma remains poorly understood. Most in vitro models that have evaluated this event have relied on monolayer transwell or microfluidic organ-on-a-chip techniques that do not account for the combined effect of all cellular layers that constitute the BBB at different sites of the Central Nervous System. There has not been direct transcytosis visualization through the BBB in mammals in vivo, and evidence comes from in vivo experiments in zebrafish. Literature is scarce on this topic, and techniques describing the mechanisms of EVs motion through the BBB are inconsistent. This review will focus on in vitro and in vivo methodologies used to evaluate EVs transcytosis, how EVs overcome this fundamental structure, and discuss potential methodological approaches for future analyses to clarify these issues. Understanding how EVs cross the BBB will be essential for their future use as vehicles in pharmacology and therapeutics.
- ItemFormation of Copper Nanoparticles Supported onto Inclusion Compounds of alpha-cyclodextrin: A New Route to Obtain Copper Nanoparticles(2010) Silva, Nataly; Moris, Silvana; Herrera Pisani, Bárbara Andrea; Diaz, Maximiliano; Kogan, Marcelo J.; Barrientos, Lorena; Yutronic, Nicolas; Jara, Paul
- ItemIn vivo micro computed tomography detection and decrease in amyloid load by using multifunctionalized gold nanorods: a neurotheranostic platform for Alzheimer's disease(Royal Soc Chemistry, 2021) Morales Zavala, Francisco; Jara Guajardo, Pedro; Chamorro Veloso, David Daniel; Riveros, Ana L.; Chandia Cristi, América Valeska; Salgado Cortés, Nicole Andrea; Pismante, Paola; Giralt, Ernest; Sanchez Navarro, Macarena; Araya, Eyleen; Vasquez, Rodrigo; Acosta, Gerardo; Albericio, Fernando; Alvarez, Alejandra R.; Kogan, Marcelo J.The development and use of nanosystems is an emerging strategy for the diagnosis and treatment of a broad number of diseases, such as Alzheimer's disease (AD). Here, we developed a neurotheranostic nanosystem based on gold nanorods (GNRs) that works as a therapeutic peptide delivery system and can be detected in vivo for microcomputed tomography (micro-CT), being a diagnostic tool. GNRs functionalized with the peptides Ang2 (a shuttle to the Central Nervous System) and D1 (that binds to the A beta peptide, also inhibiting its aggregation) allowed detecting differences in vivo between wild type and AD mice (APPswe/PSEN1dE9) 15 minutes after a single dose by micro-CT. Moreover, after a recurrent treatment for one month with GNRs-D1/Ang2, we observed a diminution of amyloid load and inflammatory markers in the brain. Thus, this new designed nanosystem exhibits promising properties for neurotheranostics of AD.
- ItemLabel-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of galectin-3, a novel cardiac biomarker(2018) Primo, Emiliano N.; Kogan, Marcelo J.; Verdejo Pinochet, Hugo; Bollo, Soledad; Rubianes, María D.; Rivas, Gustavo A.
- ItemNanoparticles for diagnosis and therapy of atherosclerosis and myocardial infarction : evolution toward prospective theranostic approaches(2018) Bejarano, Julian; Navarro-Marquez, Mario; Morales-Zavala, Francisco; Morales, Javier O.; Garcia-Carvajal, Ivonne; Araya-Fuentes, Eyleen; Flores, Yvo; Verdejo Pinochet, Hugo; Castro Gálvez, Pablo Federico; Lavandero, Sergio; Kogan, Marcelo J.
- ItemThe case for aflatoxins in the causal chain of gallbladder cancer(2016) Foerster, Claudia; Koshiol, Jill; Guerrero, Ariel R.; Kogan, Marcelo J.; Ferreccio Readi, Catterina