Browsing by Author "Kato Cardemil, Sumie Rode"
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- Item2-Methoxyestradiol inhibits progesterone-dependent tissue factor expression and activity in breast cancer cells(2010) Quezada, M.; Diaz, J.; Henriquez, S.; Bravo Castillo, María Loreto; Aranda, E.; Oliva, B.; Villalón, Manuel J.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Brosens, J. J.; Lange, C. A.; Owen, Gareth Ivor2-Methoxyestradiol (2ME) is an endogenous metabolite of 17β-estradiol with antiangiogenic and antitumor properties, although its mechanisms of action remain unclear. Progestins in hormone replacement therapy increase the risk of breast cancer. Progesteron
- Item2-Methoxyestradiol mediates apoptosis through caspase-dependent and independent mechanisms in ovarian cancer cells but not in normal counterparts(2008) Kato Cardemil, Sumie Rode; Sadarangani, Anil; Lange Smith, María Soledad; Delpiano, Ana M.; Vargas, Macarena; Brañes, Jorge; Carvajal Cabrera, Jorge Andrés; Lipkowitz, Stanley; Owen, Gareth Ivor; Cuello Fredes, Mauricio Arturo
- ItemCharacterization and phenotypic variation with passage number of cultured human endometrial adenocarcinoma cells(2008) Kato Cardemil, Sumie Rode; Villalón, Manuel J.; Cuello F., Mauricio; Owen, Gareth Ivor; Espinoza, Natalia; Lange, Soledad
- ItemCyclooxygenase-2 Blockade Is Crucial to Restore Natural Killer Cell Activity before Anti-CTLA-4 Therapy against High-Grade Serous Ovarian Cancer(2023) Gómez-Valenzuela, Fernán; Wichmann Pérez, Ignacio Alberto; Suárez, Felipe; Kato Cardemil, Sumie Rode; Ossandón, Enrique; Hermoso, Marcela; Fernández, Elmer A.; Cuello, Mauricio A.Chronic inflammation influences the tumor immune microenvironment (TIME) in high-grade serous ovarian cancer (HGSOC). Specifically, cyclooxygenase-2 (COX-2) overexpression promotes cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) expression. Notably, elevated COX-2 levels in the TIME have been associated with reduced response to anti-CTLA-4 immunotherapy. However, the precise impact of COX-2, encoded by PTGS2, on the immune profile remains unknown. To address this, we performed an integrated bioinformatics analysis using data from the HGSOC cohorts (TCGA-OV, n = 368; Australian cohort AOCS, n = 80; GSE26193, n = 62; and GSE30161, n = 45). Employing Gene Set Variation Analysis (GSVA), MIXTURE and Ecotyper cell deconvolution algorithms, we concluded that COX-2 was linked to immune cell ecosystems associated with shorter survival, cell dysfunction and lower NK cell effector cytotoxicity capacity. Next, we validated these results by characterizing circulating NK cells from HGSOC patients through flow cytometry and cytotoxic assays while undergoing COX-2 and CTLA-4 blockade. The blockade of COX-2 improved the cytotoxic capacity of NK cells against HGSOC cell lines. Our findings underscore the relevance of COX-2 in shaping the TIME and suggest its potential as a prognostic indicator and therapeutic target. Increased COX-2 expression may hamper the effectivity of immunotherapies that require NK cell effector function. These results provide a foundation for experimental validation and clinical trials investigating combined therapies targeting COX-2 and CTLA-4 in HGSOC.
- ItemDiabetic concentrations of metformin inhibit platelet-mediated ovarian cancer cell progression(2017) Erices, R.; Cubillos, Sofía; Aravena, Raúl; Santoro, Felice; Márquez, Mónica; Orellana Walden, Renán Felipe; Ramírez, Carolina; González, Pamela; Fuenzalida, Patricia; Bravo Castillo, María Loreto; Oliva, Bárbara; Kato Cardemil, Sumie Rode; Ibáñez, Carolina; Brañes, Jorge; Bravo, Erasmo; Alonso, Catalina
- ItemFunctional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor.(2020) García Cañete, Patricia; Bizama, Carolina; Rosa, Lorena.; Cerda Infante, Javier; Sánchez, Marianela.; Montecinos Acuña, Viviana; Alfaro, Francisca.; Leiva-Acevedo, Claudia.; Romero, Diego.; Kato Cardemil, Sumie RodeAbstract Background Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. Results After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. Conclusions The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.
- ItemIndependent Anti-Angiogenic Capacities of Coagulation Factors X and Xa(2014) Lange, S.; Gonzalez, I.; Pinto, M.; Arce, M.; Valenzuela Bassi, Rodrigo Andrés; Aranda, E.; Elliot, M.; Alvarez, M.; Henriquez, S.; Velasquez, Ever A.; Orge, F.; Oliva, B.; Gonzalez, P.; Villalón, Manuel J.; Cautivo Reyes, Kelly Margarita; Kalergis Parra, Alexis Mikes; Pereira, K.; Mendoza, C.; Saez, C.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Parborell, F.; Irusta, G.; Palma, V.; Allende, M.; Owen, Gareth Ivor
- ItemLeptin promotes a more aggressive behavior of ovarian cancer cells : a potential explanation for a worse prognosis in obese ovarian cancer patients(2015) Cuello F., Mauricio; Kato Cardemil, Sumie Rode; Abarzúa Catalán, L.; Delpiano, A.; García, K.; Sanhueza, C.; Ibáñez Cáceres, Carolina; Brañes Yunusic, Jorge Antonio; Castellón E.; Owen, Gareth Ivor; Trigo, C.
- ItemLeptin stimulates migration and invasion and maintains cancer stem-like properties in ovarian cancer cells : an explanation for poor outcomes in obese women(2015) Kato Cardemil, Sumie Rode; Abarzúa Catalán, Lorena; Trigo, César; Delpiano, Ana; Sanhueza, Cristobal; García, Karen; Ibáñez Cáceres, Carolina; Hormazábal, Katherine; Díaz Bórquez, Daniela Aida; Brañes Yunusic, Jorge Antonio; Castellón, Enrique; Bravo, Erasmo; Owen, Gareth Ivor; Cuello F., Mauricio
- ItemPaclitaxel-PHBV nanoparticles and their toxicity to endometrial and primary ovarian cancer cells(2013) Vilos, C.; Morales, F.; Solar, P.; Herrera, N.; González Nilo, F.; Aguayo, D.; Mendoza, H.; Bravo Castillo, María Loreto; González, P.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Owen, Gareth Ivor
- ItemPatient inflammatory status and CD4+/CD8+ intraepithelial tumor lymphocyte infiltration are predictors of outcomes in high-grade serous ovarian cancer(2018) Pinto, Mauricio P.; Balmaceda, Carlos; Bravo Castillo, María Loreto; Kato Cardemil, Sumie Rode; Villarroel, Alejandra; Owen, Gareth Ivor; Roa Strauch, Juan Carlos Enrique; Cuello F., Mauricio; Ibañez, Carolina
- ItemPlatelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells.(2015) Orellana, Renan.; Kato Cardemil, Sumie Rode; Erices, Rafaela.; Bravo Castillo, María Loreto; González Hevia, Pamela Andrea.; Oliva, Bárbara.; Cubillos Alfaro, Sofía María Jesús.; Valdivia Román, Andrés Felipe; Brañes, Jorge; Cuello F., Mauricio; Ibañez, Carolina; Barriga Cosmelli, María Isabel; Bravo, Erasmo.; Alonso, Catalina.; Bustamente, Eva.; Castellon, Enrique.; Hidalgo, Patricia.; Trigo, Cesar.; Panes Becerra, Olga Teresa; Pereira Garcés, Jaime Ignacio; Mezzano, Diego; Owen, Gareth IvorAbstract Background An increase in circulating platelets, or thrombocytosis, is recognized as an independent risk factor of bad prognosis and metastasis in patients with ovarian cancer; however the complex role of platelets in tumor progression has not been fully elucidated. Platelet activation has been associated with an epithelial to mesenchymal transition (EMT), while Tissue Factor (TF) protein expression by cancer cells has been shown to correlate with hypercoagulable state and metastasis. The aim of this work was to determine the effect of platelet-cancer cell interaction on TF and “Metastasis Initiating Cell (MIC)” marker levels and migration in ovarian cancer cell lines and cancer cells isolated from the ascetic fluid of ovarian cancer patients. Methods With informed patient consent, ascitic fluid isolated ovarian cancer cells, cell lines and ovarian cancer spheres were co-cultivated with human platelets. TF, EMT and stem cell marker levels were determined by Western blotting, flow cytometry and RT-PCR. Cancer cell migration was determined by Boyden chambers and the scratch assay. Results The co-culture of patient-derived ovarian cancer cells with platelets causes: 1) a phenotypic change in cancer cells, 2) chemoattraction and cancer cell migration, 3) induced MIC markers (EMT/stemness), 3) increased sphere formation and 4) increased TF protein levels and activity. Conclusions We present the first evidence that platelets act as chemoattractants to cancer cells. Furthermore, platelets promote the formation of ovarian cancer spheres that express MIC markers and the metastatic protein TF. Our results suggest that platelet-cancer cell interaction plays a role in the formation of metastatic foci.
- ItemProgesterone Increases Tissue Factor Gene Expression, Procoagulant Activity, and Invasion in the Breast Cancer Cell Line ZR-75-1(2005) Kato Cardemil, Sumie Rode; Pinto Paganini, Mauricio Arturo; Carvajal, Andrés.; Espinoza, Natalia.; Monso, Carolina.; Sadarangani, Anil.; Villalón, Manuel J.; Owen, Gareth Ivor
- ItemProgesterone regulation of tissue factor depends on MEK1/2 activation and requires the proline-rich site on progesterone receptor(2015) Bravo Castillo, María Loreto; Pinto, Mauricio P.; González, Ibeth; Oliva, Bárbara; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Lange, Carol A.; Owen, Gareth Ivor
- ItemRegulation of GLUT3 and glucose uptake by the cAMP signalling pathway in the breast cancer cell line ZR‐75(2008) Meneses, Ana Maria.; Kato Cardemil, Sumie Rode; Owen, Gareth Ivor
- ItemTécnica para cultivar y expandir células de cáncer extraídas de fluido ascitico y su exposición a concentraciones específicas de regímenes de quimioterapia (Chile, concesión n° 55061)Owen, Gareth Ivor; Bravo Castillo, María Loreto; González, Pamela; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Orellana Walden, Renán Felipe
- ItemThe impact on high-grade serous ovarian cancer of obesity and lipid metabolism-related gene expression patterns: the underestimated driving force affecting prognosis(2017) Cuello F., Mauricio; Kato Cardemil, Sumie Rode; Liberona, Francisca
- ItemTissue factor is regulated by epidermal growth factor in normal and malignant human endometrial epithelial cells(2005) Kato Cardemil, Sumie Rode; Pinto Paganini, Mauricio Arturo; Carvajal, Andrés.; Espinoza, Natalia.; Monsó, Carolina.; Bravo Castillo, María Loreto; Villalón, Manuel J.; Cuello F., Mauricio; Owen, Gareth Ivor
- ItemTRAIL mediates apoptosis in cancerous but not normal primary cultured cells of the human reproductive tract(2007) Sadarangani, A.; Kato Cardemil, Sumie Rode; Villalón, Manuel J.; Owen, Gareth Ivor