Browsing by Author "Infante, Rodrigo"

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    Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: an approach to the appropriate dosage for breeding purposes
    (UNIV NACIONAL CUYO, FAC CIENCIAS AGRARIAS, 2012) Aros, Danilo; Valdes, Santiago; Olate, Eduardo; Infante, Rodrigo
    Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes.
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    Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness
    (2023) Olmedo, Patricio; Zepeda, Baltasar; Delgado-Rioseco, Joaquin; Leiva, Carol; Moreno, Adrian A.; Sagredo, Karen; Blanco-Herrera, Francisca; Pedreschi, Romina; Infante, Rodrigo; Meneses, Claudio; Campos-Vargas, Reinaldo
    Chilling injury is a physiological disorder caused by cold storage in peaches and nectarines. The main symptom of chilling injury is mealiness/wooliness, described as a lack of juice in fruit flesh. In this work, we studied two nectarine varieties (Andes Nec-2 and Andes Nec-3) with contrasting susceptibility to mealiness after cold storage. A non-targeted metabolomic analysis was conducted by GC-MS to understand if changes in metabolite abundance are associated with nectarine mealiness induced by cold storage. Multivariate analyses indicated that in unripe nectarines, cold storage promoted a higher accumulation of amino acids in both varieties. Interestingly, for ripe nectarines, cold storage induced an accumulation of fewer amino acids in both varieties and showed an increased abundance of sugars and organic acids. A pathway reconstruction of primary metabolism revealed that in ripe nectarines, cold storage disrupted metabolite abundance in sugar metabolism and the TCA cycle, leading to a differential accumulation of amino acids, organic acids, and sugars in mealy and juicy nectarines.