Browsing by Author "Huidobro-Toro, Juan Pablo"
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- ItemClonidine‐induced nitric oxide‐dependent vasorelaxation mediated by endothelial α2‐adrenoceptor activation(2001) Figueroa, Xavier; Boric P., Mauricio; Huidobro-Toro, Juan Pablo
- ItemD-myo-Inositol 1,2,6-triphosphate blocks neuropeptide Y-induced facilitation of noradrenaline-evoked vasoconstriction of the mesenteric bed(1993) Donoso Gomien, María Verónica; Boric P., Mauricio; Huidobro-Toro, Juan Pablo
- ItemEffect of endothelin on total and regional coronary resistence and on myocardial contractility(1991) Domenech, Raúl; González Aldunate, Rolando; Huidobro-Toro, Juan Pablo
- ItemEndothelin reduces microvascular blood flow acting on arterioles and venules of the hamster cheek pouch(1990) Boric P., Mauricio; Donoso Gomien, María Verónica; Huidobro-Toro, Juan Pablo
- ItemMolecular mechanism of cGMP-mediated smooth muscle relaxation(2000) Carvajal C., Jorge A.; Germain Aravena, Alfredo M.; Huidobro-Toro, Juan Pablo
- ItemNeuropeptide Y is a vasoconstrictor and adrenergic modulator in the hamster microcirculation by acting on neuropeptide Y1 and Y2 receptors(1995) Boric P., Mauricio; Huidobro-Toro, Juan Pablo
- ItemNeurosteroids differentially modulate P2X(4) ATP-gated channels through non-genomic interactions(2009) Codocedo Henríquez, Juan Francisco; Huidobro-Toro, Juan Pablo
- ItemNucleotide P2Y1 receptor regulates EGF receptor mitogenic signaling and expression in epithelial cells(2007) Buvinic, Sonja; Bravo-Zehnder, Marcela; Boyer, Jose Luis; Huidobro-Toro, Juan Pablo; Gonzalez, AlfonsoEpidermal growth factor receptor ( EGFR) function is transregulated by a variety of stimuli, including agonists of certain G-protein-coupled receptors (GPCRs). One of the most ubiquitous GPCRs is the P2Y(1) receptor (P2RY1, hereafter referred to as P2Y(1)R) for extracellular nucleotides, mainly ADP. Here, we show in tumoral HeLa cells and normal FRT epithelial cells that P2Y(1)R broadcasts mitogenic signals by transactivating the EGFR. The pathway involves PKC, Src and cell surface metalloproteases. Stimulation of P2Y(1)R for as little as 1560 minutes triggers mitogenesis, mirroring the half-life of extracellular ADP. Apyrase degradation of extracellular nucleotides and drug inhibition of P2Y(1)R, both reduced basal cell proliferation of HeLa and FRT cells, but not MDCK cells, which do not express P2Y(1)R. Thus, cell-released nucleotides constitute strong mitogenic stimuli, which act via P2Y(1)R. Strikingly, MDCK cells ectopically expressing P2Y(1)R display a highly proliferative phenotype that depends on EGFR activity associated with an increased level of EGFR, thus disclosing a novel aspect of GPCR-mediated regulation of EGFR function. These results highlight a role of P2Y(1)R in EGFR-dependent epithelial cell proliferation. P2Y(1)R could potentially mediate both trophic stimuli of basally released nucleotides and first-line mitogenic stimulation upon tissue damage. It could also contribute to carcinogenesis and serve as target for antitumor therapies.
- ItemRise in endothelium-derived NO after stimulation of rat perivascular sympathetic mesenteric nerves(1999) Boric P., Mauricio; Figueroa, Xavier; Huidobro-Toro, Juan Pablo
- ItemStoichiometry and conditional stability constants of Cu(II) or Zn(II) clioquinol complexes; implications for Alzheimer's and Huntington's disease therapy(2007) Ferrada, Evandro; Arancibia Moya, Verónica; Loeb Luschow, Bárbara; Huidobro-Toro, Juan Pablo
- ItemThe hypolipidemic drug metabolites nafenopin‐CoA and ciprofibroyl‐CoA are competitive P2Y1 receptor antagonists(2003) Coddou, Claudio; Bronfman A., Miguel L.; Huidobro-Toro, Juan Pablo
- ItemThe Role of Adenosine A(2a) and A(3) Receptors on the Differential Modulation of Norepinephrine and Neuropeptide y Release From Peripheral Sympathetic Nerve Terminals(2006) Donoso Gomien, María Verónica; Aedo, Felipe; Huidobro-Toro, Juan Pablo