Browsing by Author "González, M."

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    An inactivated SARS-CoV-2 vaccine is safe and induces humoral and cellular immunity against virus variants in healthy children and adolescents in Chile
    (2022) Soto, J.A.; Melo-González, F.; Gutierrez-Vera, C.; Schultz, B.M.; Berríos-Rojas, R.V.; Rivera-Pérez, D.; Piña-Iturbe, A.; Hoppe-Elsholz, G.; Duarte, L.F.; Vázquez, Y.; Moreno-Tapia, D.; Ríos, M.; Palacios, P.A.; Garcia-Betancourt, R.; Santibañez, Á.; Mendez, C.; Diethelm-Varela, B.; Astudillo, P.; Calvo, M.; Cárdenas, A.; González, M.; Goldsack, M.; Gutiérrez, V.; Potin, M.; Schilling, A.; Tapia, L.I.; Twele, L.; Villena, R.; Grifoni, A.; Sette, A.; Weiskopf, D.; Fasce, R.A.; Fernández, J.; Mora, J.; Ramírez, E.; Gaete-Argel, A.; Acevedo, M.; Valiente-Echeverría, F.; Soto-Rifo, R.; Retamal-Díaz, A.; Muñoz-Jofré, N.; Meng, X.; Xin, Q.; Alarcón-Bustamante, E.; González-Aramundiz, J.V.; Le Corre, N.; Álvarez, M.J.; González, P.A.; Abarca, K.; Perret, C.; Carreño, L.J.; Kalergis, A.M.; Bueno, S.M.
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    Cetoacidosis diabética : Casuística 2008-2012, epidemiología y fisiopatología
    (2014) Olmos Coelho, Pablo Roberto; Donoso Henríquez, Aníbal Tomás; Arab Verdugo, Juan Pablo; Niklitschek, I.; Mertens, N.; Arce, E.; Lemus, R.; Serrano Larrea, Valentina; Grassi Corrales, Bruno; Strodthoff Simunovic, Kristel; Abbott Cáceres, Eduardo Francisco; Aizman, Andrés; González, M.
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    Effect of Ni Metal Content on Emulsifying Properties of Ni/CNTox Catalysts for Catalytic Conversion of Furfural in Pickering Emulsions
    (2021) Herrera Hernández, Carla Pía; Pinto Neira, Josefa; Fuentealba Patiño, Denis Alberto; Sepúlveda, C.; Rosenkranz, Andreas; García-Fierro, J. L.; González, M.; Escalona, Néstor
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    Equilibrative nucleoside transporter 1 expression is downregulated by hypoxia in human umbilical vein endothelium
    (2005) Casanello, P.; Torres, A.; Sanhueza, F.; González, M.; Farías, M.; Gallardo, V.; Pastor-Anglada, M.; San Martín, R.; Sobrevía Luarte, Luis Alberto
    Reduced oxygen level (hypoxia) induces endothelial dysfunction and release of the endogenous nucleosideadenosine. Human umbilical vein endothelium (HUVEC) function in an environment with 3% to 5% O2and exhibitefficient adenosine membrane transport via human equilibrative nucleoside transporters 1 (hENT1). We studied whetheradenosine transport and hENT1 expression are altered by hypoxia in HUVEC. Hypoxia (0 to 24 hours, 2% and 1% O2)reduced maximal hENT1-adenosine transport velocity (Vmax) and maximal nitrobenzylthionosine (NBMPR, a high-affinity hENT1 protein ligand) binding, but increased extracellular adenosine concentration. Hypoxia also reducedhENT1 protein and mRNA levels, effects unaltered byN_x0001_-nitro-L-arginine methyl ester (L-NAME, nitric oxide synthase[NOS] inhibitor) or PD-98059 (inhibitor of mitogen-activated protein kinase kinase 1 and 2 [MEK1/2]). Hypoxiareduced endothelial NOS (eNOS) activity and eNOS phosphorylation at Ser1177, but increased eNOS protein level.Hypoxia increased (1 to 3 hours), but reduced (24 hours) p42/44mapkphosphorylation. Thus, hypoxia-increasedextracellular adenosine may result from reduced hENT1-adenosine transport in HUVEC. Hypoxia effect seems not toinvolve NO, but p42/44mapkmay be required for the relatively rapid effect (1 to 3 hours) of hypoxia. These results couldbe important in diseases where the fetus is exposed to intrauterine environments poor in oxygen, such as intrauterinegrowth restriction, or where adenosine transport is altered, such as gestational diabetes.
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    Estrategias de intervención para mejorar la calidad de vida en pacientes con insuficiencia cardíaca
    (2013) González, M.; Pedrero, V.
    Objetivos: Conocer las características de las estrategias de intervención multidisciplinarias implementadas por el equipo de salud que mejoran la calidad de vida de los pacientes con insuficiencia cardíaca. Métodos: Se consultó las bases de datos Pubmed, Cochrane library, Proquest y CINHAL entre mayo y junio del 2011, encontrándose 99 artículos de los cuales se seleccionaron 40 (estudios randomizados controlados, metanálisis y revisiones sistemáticas) publicados en los últimos 10 años. Resultados:Entre las variables que tienen mayor efecto sobre la calidad de vida de los pacientes con insuficiencia cardíaca se encuentran: intervenciones educativas (reconocimiento de signos y síntomas, adherencia al tratamiento farmacológico, cambios del estilo de vida, peso diario, etc.); consejería telefónica o video llamadas (ajuste de terapia diurética); y actividad física (prueba de caminata de 6 minutos, ejercicios aeróbicos, etc.). La implementación de estrategias de intervención multidisciplinarias tiene un efecto positivo en el incremento de la calidad de vida. Existe una relación significativa entre las sub-escalas de calidad de vida (funcionamiento físico, salud general y mental), y del puntaje total de la dimensión física con el riesgo de readmisión hospitalaria. Conclusión: Intervenciones multidisciplinarias de tipo educativo y aquellas orientadas a mejorar la condición física tienen efecto positivo en mejorar la calidad de vida de los pacientes con Insuficiencia Cardíaca. Ello se relaciona con una disminución del riesgo de re-hospitalización.
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    Hepatic metabolic response to restricted copper intake in a Niemann-Pick C murine model
    (2014) Argueello, G.; Martinez, P.; Peña, J.; Chen, O.; Platt, F.; Zanlungo Matsuhiro, Silvana; González, M.
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    In vitro and in silico evaluations of new aryloxy-1,4-naphthoquinones as anti-Trypanosoma cruzi agents
    (2020) González, A.; Becerra, Natalia; Kashif, M.; González, M.; Cerecetto, H.; Aguilera, E.; Nogueda-Torres, B.; Chacón-Vargas, K. F.; Zarate-Ramos, J. J.; Salas Sánchez, Cristián Osvaldo; Castillo-Velázquez, U; Rivera, G.; Vázquez, K.
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    Inactivated Vaccine-Induced SARS-CoV-2 Variant-Specific Immunity in Children
    (2022) Soto, J.A.; Melo-González, F.; Gutierrez-Vera, C.; Schultz, B.M.; Berríos-Rojas, R.V.; Rivera-Pérez, D.; Piña-Iturbe, A.; Hoppe-Elsholz, G.; Duarte, L.F.; Vázquez, Y.; Moreno-Tapia, D.; Ríos, M.; Palacios, P.A.; Garcia-Betancourt, R.; Santibañez, Á.; Pacheco, G.A.; Mendez, C.; Andrade, C.A.; Silva, P.H.; Diethelm-Varela, B.; Astudillo, P.; Calvo, M.; Cárdenas, A.; González, M.; Goldsack, M.; Gutiérrez, V.; Potin, M.; Schilling, A.; Tapia, L.I.; Twele, L.; Villena, R.; Grifoni, A.; Sette, A.; Weiskopf, D.; Fasce, R.A.; Fernández, J.; Mora, J.; Ramírez, E.; Gaete-Argel, A.; Acevedo, M.L.; Valiente-Echeverría, F.; Soto-Rifo, R.; Retamal-Díaz, A.; Muñoz-Jofré, N.; Meng, X.; Xin, Q.; Alarcón-Bustamante, E.; González-Aramundiz, J.V.; Le Corre, N.; Álvarez-Figueroa, M.J.; González, P.A.; Abarca, K.; Perret, C.; Carreño, L.J.; Bueno, S.M.; Kalergis, A.M.
    Multiple vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been evaluated in clinical trials. However, trials addressing the immune response in the pediatric population are scarce. The inactivated vaccine CoronaVac has been shown to be safe and immunogenic in a phase 1/2 clinical trial in a pediatric cohort in China. Here, we report interim safety and immunogenicity results of a phase 3 clinical trial for CoronaVac in healthy children and adolescents in Chile. Participants 3 to 17 years old received two doses of CoronaVac in a 4-week interval until 31 December 2021. Local and systemic adverse reactions were registered for volunteers who received one or two doses of CoronaVac. Whole-blood samples were collected from a subgroup of 148 participants for humoral and cellular immunity analyses. The main adverse reaction reported after the first and second doses was pain at the injection site. Four weeks after the second dose, an increase in neutralizing antibody titer was observed in subjects relative to their baseline visit. Similar results were found for activation of specific CD4+ T cells. Neutralizing antibodies were identified against the Delta and Omicron variants. However, these titers were lower than those for the D614G strain. Importantly, comparable CD4+ T cell responses were detected against these variants of concern. Therefore, CoronaVac is safe and immunogenic in subjects 3 to 17 years old, inducing neutralizing antibody secretion and activating CD4+ T cells against SARS-CoV-2 and its variants. (This study has been registered at ClinicalTrials.gov under no. NCT04992260.) IMPORTANCE This work evaluated the immune response induced by two doses of CoronaVac separated by 4 weeks in healthy children and adolescents in Chile. To date, few studies have described the effects of CoronaVac in the pediatric population. Therefore, it is essential to generate knowledge regarding the protection of vaccines in this population. Along these lines, we reported the anti-S humoral response and cellular immune response to several SARS-CoV-2 proteins that have been published and recently studied. Here, we show that a vaccination schedule consisting of two doses separated by 4 weeks induces the secretion of neutralizing antibodies against SARS-CoV-2. Furthermore, CoronaVac induces the activation of CD4+ T cells upon stimulation with peptides from the proteome of SARS-CoV-2. These results indicate that, even though the neutralizing antibody response induced by vaccination decreases against the Delta and Omicron variants, the cellular response against these variants is comparable to the response against the ancestral strain D614G, even being significantly higher against Omicron.
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    Ki67 dynamic predicts endocrine sensitivity in estrogen receptor-positive/ HER2-negative breast cancer patients undergoing preoperative endocrine therapy
    (2025) Gómez-Bravo, R.; Walbaum, B.; Bergamino, M.; Martínez-Sáez, O.; Schettini, F.; Seguí, E.; García-Fructuoso, I.; Pascual, T.; Chic, N.; González, M.; Rodríguez, A.; Rey, M.; Giménez-Xavier, P.; Blasco, P.; Castillo, O.; Galván, P.; Sanfeliu, E.; González-Farré, B.; Vidal, M.; Adamo, B.; Brasó-Maristany, F.; Prat, A.; Muñoz, M.
    Background: Early decrease in Ki67 after a short preoperative course of endocrine therapy (ET) has shown prognostic and predictive value in clinical research, but its applicability and reproducibility in routine clinical practice remain largely unknown. We therefore assessed on-treatment Ki67 changes following a short preoperative ET and its association with biological variables, such as intrinsic subtype and risk of recurrence (ROR), plus long-term outcomes, in a real-world cohort of patients with early estrogen receptor-positive, human epidermal growth factor receptor 2-negative (ER+/HER2-negative) breast cancer. Methods: We conducted a retrospective, registry-based analysis of 230 consecutive patients with early ER+/HER2− breast cancer treated as per standard clinical care at the Breast Unit of the Clinic Barcelona Comprehensive Cancer Center between 2014 and 2023. All patients received preoperative ET, tamoxifen, or an aromatase inhibitor (AI), for 2-12 weeks before surgery. Clinical and pathological variables were collected and stratified by Ki67 response: “responders” (post-treatment Ki67 0% to 10%) and “complete cell cycle arrest (CCCA) responders” (Ki67 ≤2.7%). PAM50/Prosigna was used to determine intrinsic subtypes and ROR-score. Event-free survival was estimated using Kaplan—Meier curves, and associations were tested using Cox proportional hazards regression. Results: The median duration of preoperative ET was 5 weeks (min-max range, 2-12 weeks). Overall, 196 patients (85.2%) met the Ki67 response criterion and 111 (48.3%) achieved CCCA. Response rates were significantly higher in postmenopausal compared with premenopausal women (P = 0.004). Notably, 95.6% of postmenopausal patients received an AI, whereas all premenopausal women were treated with tamoxifen. Additionally, response varied by intrinsic subtype, favoring Luminal A tumors (P = 0.047). In multivariable models, postmenopausal status and higher baseline ER expression were independently associated with both Ki67 response and CCCA, whereas a lower baseline ROR-score predicted CCCA. After a median follow-up of 47 months, CCCA was associated with significantly improved event-free survival [hazard ratio (HR) = 0.19; 95% CI (confidence interval) 0.05-0.72; P value = 0.012]. Conclusion: In routine practice, a short course of preoperative ET yields substantial reductions in tumor proliferation. Early assessment of Ki67 suppression offers a readily accessible indicator of endocrine sensitivity, and achieving CCCA identifies patients who have a more favorable prognosis and thus are potentially eligible to de-escalate in treatment strategies.
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    Maternal thyroid profile in first and second trimester of pregnancy is correlated with gestational diabetes mellitus through machine learning
    (2021) Araya, J.; Rodríguez, A.; Lagos SanMartin, K.; Mennickent, D.; Gutiérrez Vega, S.; Ortega Contreras, B.; Valderrama Gutiérrez, B.; González, M.; Farías Jofré, Marcelo Enrique; Guzmán Gutiérrez, E.
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    New aryloxy-quinone derivatives as potential anti-Chagasic agents : synthesis, trypanosomicidal activity, electrochemical properties, pharmacophore elucidation and 3D-QSAR analysis
    (2015) Vázquez, K.; Espinosa Bustos, Christian Marcelo; Soto, J.; Tapia, R.; Varela, J.; Birriel, E.; Segura Segura, Rodrigo; Pizarro, J.; Cerecetto, H.; González, M.; Paulino, M.; Salas, C.
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    Overexpression of GlyI and GlyII genes in transgenic tomato (Solanum lycopersicum Mill.) plants confers salt tolerance by decreasing oxidative stress
    (2013) Alvarez M.; Inostroza Blancheteau, C.; Timmermann, T.; González, M.; Arce Johnson, Jorge Patricio
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    Photosynthetic and Genetic Adaptations Underpinning the Resilience of Cistanthe longiscapa in the Atacama Desert
    (2024) Sandoval-Ibáñez, O.; Tapia-Reyes, P.; Riveros, Anibal; Yusta, R.; Chang, S.; Ossa, P.; Nilo-Poyanco, R.; Moreno, A.A.; Miquel, A.; Almeida, A.M.; Zurita-Silva, A.; Orellana, D.; Baeza, C.; Blanco-Herrera, F.; Genova, A.D.; Allende, M.L.; González, M.; Maass, A.; Gutierrez Alliende, Rodrigo Hernan; Meneses Araya, Claudio Antonio
    The Atacama Desert is one of the most hostile environments for life. However, the plant species Cistanthe longiscapa (C. longiscapa) completes its life cycle in the Atacama Desert after sporadic rainfall. Physiological analyses under controlled environmental conditions revealed superior photosynthetic performance, better light acclimation mechanisms, and larger accumulation of photosystem II in C. longiscapa compared to its mesophilic sister species. C. longiscapa shows evolutionary expansions in gene families related to DNA repair, photosynthesis, and protein homeostasis. In addition, we observed substantial gene duplication and polymorphic variations between coastal and inland populations in the Atacama Desert. Finally, our assembled mitochondrial genome provides genetic information for all DNA-containing compartments of C. longiscapa. Diurnal oscillations of malic acid and time-resolved transcriptome analyses of plants harvested in the Atacama Desert indicate that C. longiscapa engages in CAM metabolism. We observed significant differences in transcripts encoding plastid-localized proteins, including those involved in carbon metabolism, light harvesting, and photoprotection, highlighting the critical role of chloroplasts in the adaptation of C. longiscapa to the Atacama Desert. Our study provides physiological and genetic evidence for the adaptations of C. longiscapa and advances our understanding of how plants can cope with extreme environmental conditions.
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    Preocupación por el bienestar de residentes, un tema presente en la Conferencia Latinoamericana en Educación de Residentes (LACRE) 2017
    (2017) Bitrán Carreño, Marcela; González, M.; Nitsche P.; Zúñiga Parada, Denisse Alejandra; Riquelme, A.
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    Prevalence of emotional symptoms in Chilean oncology patients before the start of chemotherapy: Potential of the distress thermometer as an ultra-brief screening instrument
    (2014) Calderón, J.; Campla, C.; D'Aguzan, N.; Barraza, S.; Padilla, O.; Sánchez, C.; Palma, S.; González, M.
    Emotional distress (ED) is greater for oncology patients in comparison with the general population, and this has implications for the quality of life of the patient and his/her family, adherence to the treatment, and eventually, survivorship. In general, the detection of these symptoms is low, which explains the need for detection systems appropriate to the clinical reality of the oncology team. The objective of this study is to evaluate for the first time the usefulness of an ultra-brief screening instrument [distress thermometer (DT)], in a group of Chilean oncology patients. A total of 166 outpatients were evaluated at the Cancer Center of the Pontificia Universidad Católica de Chile, before starting chemotherapy. Two screening instruments were applied: Hospital Anxiety and Depression Scale (HADS) and DT. The application of HADS resulted in a prevalence of 32.7% of anxiety symptoms (HADS-A ≥ 8), 15.7% of depression symptoms (HADS-D ≥ 8), and 39.8% had a total score of HADS-T ≥ 11. The DT resulted in the prevalence of 32.5% of distress or ED (DT ≥ 5). The validity of the DT was evaluated as a screening tool in comparison with HADS, observing, in relation to the anxiety scale (HADS-A), a sensitivity of 88.9% and specificity of 78.4% (DT ≥ 4); depression (HADS-D), a sensitivity of 69.2% and specificity of 74.3% (DT ≥ 5); and in relation to the total scale (HADS-T), a sensitivity of 68.2% and specificity of 73.0% (DT ≥ 4). This study demonstrates the elevated prevalence of emotional symptoms in Chilean oncology patients, before the start of chemotherapy, and confirms the potential of the DT as a brief screening instrument with easy application. The DT will allow the clinician to increase the detection threshold in the Chilean oncology population, intervene in a timely manner, and contribute to the comprehensive handling of the oncology patient without affecting the time needed for assistance.
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    Prevalence of eosinophilic esophagitis : A multicenter study on a pediatric population evaluated at thirty-six Latin American gastroenterology centers
    (2019) Pierre, R.; Vieira, M.; Vázquez, R.; Ninomiya, I.; Messere, G.; Daza, W.; Dadan, S.; Higuera, M.; Harris D., Paul R.; Gana Ansaldo, Juan Cristóbal; Sifontes, L.; Rodríguez, M.; Vásquez, M.; González, M.; Rivera, J.; Gonzales, J.; Angulo, D.; Cetraro, M. D.; Del Compared, M.; López, K.; Navarro, D.; Calva, R.; Wagener, M.; Zablah, R.; Carias, A.; Calderón, O.; Vera Chamorro, J. F.; Toca, M. C.; Dewaele, M. R.; Iglesias, C.; Delgado, L.; León, K.; Hassan, I.; Ussher, F.; Follett, F.; Bernedo, V.; Grinblat, V.; Agüero, N.; Oviedo, C.; García, A. G.; Salazar, A.; Coello, P.; Furnes, R.; Menchaca, M.; Fernández, M.; Khoury, A.; Rojo, C.; Fernández, S.; Morao, C.
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    Pronóstico de los enfermos con enfermedad pulmonar obstructiva crónica reagudizada que precisan ventilación mecánica
    (2006) Frutos-Vivar, Fernando; Esteban, Andrés; Anzueto, A.; Apezteguia, C.; González, M.; Bugedo Tarraza, Guillermo; d´Empaire, G.; Pálizas, F.; Elizalde, J.; David, C. M.; Rodrigo, C.
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    QUBIC: Using NbSi TESs with a Bolometric Interferometer to Characterize the Polarization of the CMB
    (2020) Piat, M.; Belier, B.; Berge, L.; Bleurvacq, N.; Chapron, C.; Dheilly, S.; Dumoulin, L.; González, M.; Grandsire, L.; Puddu, Roberto; Hamilton, J. C.; Henrot Versille, S.; Hoang, D. T.; Marnieros, S.; Marty, W.; Montier, L.; Olivieri, E.; Oriol, C.; Perbost, C.; Prele, D.; Rambaud, D.; Salatino, M.; Stankowiak, G.; Thermeau, J. P.; Torchinsky, S.; Voisin, F.; Ade, P.; Alberro, J. G.; Almela, A.; Amico, G.; Arnaldi, L. H.; Auguste, D.; Aumont, J.; Azzoni, S.; Banfi, S.; Battaglia, P.; Battistelli, E. S.; Bau, A.; Bennett, D.; Bernard, J. P.; Bersanelli, M.; Bigot Sazy, M. A.; Bonaparte, J.; Bonis, J.; Bottani, A.; Bunn, E.; Burke, D.; Buzi, D.; Buzzelli, A.; Cavaliere, F.
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    Reducing mine water use in arid areas through the use of a byproduct road dust suppressant
    (2019) González Rojas, Alvaro; Aitken, D.; Heitzer, C.; López, C.; González, M.