Browsing by Author "Garg, A."

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    Absence of AGPAT2 impairs brown adipogenesis, increases IFN stimulated gene expression and alters mitochondrial morphology
    (2020) Tapia Ossa, Pablo José; Figueroa Toledo, Ana María; Eisner Sagüés, Verónica Raquel; González Hódar, Lila Alejandra; Robledo Plaza, Fermín Alberto; Agarwal, AK; Garg, A.; Cortés Mora, Víctor Antonio
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    Decision-Making about Healthcare Related Tests and Diagnostic Strategies: User Testing of GRADE Evidence Tables
    (2015) Mustafa, R.; Wiercioch, W.; Santesso, N.; Cheung, A.; Prediger, B.; Baldeh, T.; Carrasco, A.; Brignardello, R.; Neumann Burotto, Gonzalo Ignacio; Bossuyt, P.; Garg, A.; Lelgemann, M.; Buhler, D.; Brozek, J.; Schunemann, H.
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    Decreased caveolae in AGPAT2 lacking adipocytes is independent of changes in cholesterol or sphingolipid levels: A whole cell and plasma membrane lipidomic analysis of adipogenesis
    (2021) González-Hódar, L.; McDonald, J.G.; Vale, G.; Thompson, B.M.; Figueroa, A.-M.; Tapia, P.J.; Robledo, F.; Agarwal, A.K.; Garg, A.; Horton, J.D.; Cortés, V.
    Background Adipocytes from lipodystrophic Agpat2−/− mice have impaired adipogenesis and fewer caveolae. Herein, we examined whether these defects are associated with changes in lipid composition or abnormal levels of caveolae-associated proteins. Lipidome changes were quantified in differentiated Agpat2−/− adipocytes to identify lipids with potential adipogenic roles. Methods Agpat2−/− and wild type brown preadipocytes were differentiated in vitro. Plasma membrane was purified by ultracentrifugation. Number of caveolae and caveolae-associated proteins, as well as sterol, sphingolipid, and phospholipid lipidome were determined across differentiation. Results Differentiated Agpat2−/− adipocytes had decreased caveolae number but conserved insulin signaling. Caveolin-1 and cavin-1 levels were equivalent between Agpat2−/− and wild type adipocytes. No differences in PM cholesterol and sphingolipids abundance were detected between genotypes. Levels of phosphatidylserine at day 10 of differentiation were increased in Agpat2−/− adipocytes. Wild type adipocytes had increased whole cell triglyceride, diacylglycerol, phosphatidylglycerol, phosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, and trihexosyl ceramide, and decreased 24,25-dihydrolanosterol and sitosterol, as a result of adipogenic differentiation. By contrast, adipogenesis did not modify whole cell neutral lipids but increased lysophosphatidylcholine, sphingomyelin, and trihexosyl ceramide levels in Agpat2−/− adipocytes. Unexpectedly, adipogenesis decreased PM levels of main phospholipids in both genotypes. Conclusion In Agpat2−/− adipocytes, decreased caveolae is not associated with changes in PM cholesterol nor sphingolipid levels; however, increased PM phosphatidylserine content may be implicated. Abnormal lipid composition is associated with the adipogenic abnormalities of Agpat2 −/− adipocytes but does not prevent insulin signaling.
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    HIGH-AMPLITUDE delta-SCUTIS IN THE LARGE MAGELLANIC CLOUD
    (2010) Garg, A.; Clocchiatti, Alejandro; Minniti, D.