Browsing by Author "Fernandez Galilea, Marta"
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- ItemAGPAT2 deficiency impairs adipogenic differentiation in primary cultured preadipocytes in a non-autophagy or apoptosis dependent mechanism(2015) Fernandez Galilea, Marta; Tapia, Pablo; Cautivo Reyes, Kelly Margarita; Morselli, Eugenia; Cortés Mora, Víctor Antonio
- ItemEffects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes(ELSEVIER SCIENCE BV, 2016) Prieto Hontoria, Pedro L.; Perez Matute, Patricia; Fernandez Galilea, Marta; Lopez Yoldi, Miguel; Sinal, Christopher J.; Alfredo Martinez, J.; Moreno Aliaga, Maria J.Chemerin is a novel adipokine associated with obesity and insulin resistance. a-Lipoic acid (alpha-LA) has shown beneficial properties on diabetes and obesity. The aim of this study was to examine the effects of alpha-LA on chemerin production in adipocytes in absence or presence of TNF-alpha, insulin and AICAR. The potential signaling pathways involved in alpha-LA effects on chemerin were also analyzed. alpha-LA actions on chemerin were tested in differentiated 3T3-L1 adipocytes and in some cases in human subcutaneous and omental adipocytes. Chemerin mRNA levels were measured by RT-PCR and the amount of chemerin secreted to culture media was determined by ELISA. alpha-LA induced a concentration-dependent inhibition on both chemerin secretion and mRNA levels in 3T3-L1 adipocytes. The AMPK activator AICAR and the PI3K inhibitor LY294002 dramatically abrogated both chemerin secretion and gene expression, and further potentiated the inhibitory effect of alpha-LA on chemerin secretion. Insulin was able to partially reverse the inhibitory action of alpha-LA on chemerin secretion. alpha-LA also reduced basal chemerin secretion in both subcutaneous and omental adipocytes from overweight/obese subjects. Moreover, alpha-LA was able to abolish the stimulatory effects of the pro-inflammatory cytokine TNF-alpha on chemerin secretion. Our data demonstrated the ability of alpha-LA to inhibit chemerin production, an adipokine associated to obesity and metabolic syndrome, suggesting that the reduction of chemerin could contribute to the antiobesity/antidiabetic properties described for alpha-LA. 2015 Elsevier B.V. All rights reserved.
- ItemEffects of dietary supplementation with EPA and/or -lipoic acid on adipose tissue transcriptomic profile of healthy overweight/obese women following a hypocaloric diet(WILEY, 2017) Huerta, Ana E.; Prieto Hontoria, Pedro L.; Fernandez Galilea, Marta; Escote, Xavier; Alfredo Martinez, J.; Moreno Aliaga, Maria J.In obesity, the increment of adiposity levels disrupts the whole body homeostasis, promoting an over production of oxidants and inflammatory mediators. The current study aimed to characterize the transcriptomic changes promoted by supplementation with eicosapentaenoic acid (EPA, 1.3 g/day), -lipoic acid (0.3 g/day), or both (EPA+-lipoic acid, 1.3 g/day+0.3 g/day) in subcutaneous abdominal adipose tissue from overweight/obese healthy women, who followed a hypocaloric diet (30% of total energy expenditure) during ten weeks, by using a microarray approach. At the end of the intervention, a total of 33,297 genes were analyzed using Affymetrix GeneChip arrays. EPA promoted changes in extracellular matrix remodeling gene expression, besides a rise of genes associated with either chemotaxis or wound repair. -Lipoic acid decreased expression of genes related with cell adhesion and inflammation. Furthermore, -lipoic acid, especially in combination with EPA, upregulated the expression of genes associated with lipid catabolism while downregulated genes involved in lipids storage. Together, all these data suggest that some of the metabolic effects of EPA and -lipoic acid could be related to their regulatory actions on adipose tissue metabolism. (c) 2016 BioFactors, 43(1):117-131, 2017