Browsing by Author "Fardella B., Carlos"

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    Age-Related Changes in 11 beta-Hydroxysteroid Dehydrogenase Type 2 Activity in Normotensive Subjects
    (2013) Campino Johnson, María del Carmen; Martínez Aguayo, Alejandro Gregorio; Baudrand Biggs, René; Carvajal Maldonado, Cristián Andrés; Aglony Imbarack, Marlene Elizabeth; García Bruce, Hernán; Padilla Pérez, Oslando; Kalergis Parra, Alexis Mikes; Fardella B., Carlos
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    Aldosterona e IL-17 en la génesis de la hipertensión arterial mineralocorticoídea, un estudio ex vivo
    (2016) Vecchiola Cárdenas, Andrea Paola; Cristóbal Fuentes, Z.; Muñoz Durango, Natalia; Tapia Castillo, Alejandra; González Gómez, Luis M.; Baudrand Biggs, René; Carvajal Maldonado, Cristián Andrés; Campino Johnson, María del Carmen; Kalergis Parra, Alexis Mikes; Carlos, F.; Lagos, A.; Fardella B., Carlos; Vecchiola Cárdenas, Andrea Paola; Cristóbal Fuentes, Z.; Muñoz Durango, Natalia; Tapia Castillo, Alejandra; González Gómez, Luis M.; Baudrand Biggs, René; Carvajal Maldonado, Cristián Andrés; Campino Johnson, María del Carmen; Kalergis Parra, Alexis Mikes; Carlos, F.; Lagos, A.; Fardella B., Carlos
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    Aldosterone as a modulator of immunity: implications in the organ damage
    (2011) Herrada, A.; Campino Johnson, María del Carmen; Fardella B., Carlos; Kalergis Parra, Alexis Mikes
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    Aldosterone Production and Signaling Dysregulation in Obesity
    (2016) Vecchiola Cárdenas, Andrea Paola; Lagos, C.; Carvajal Maldonado, Cristián Andrés; Baudrand Biggs, René; Fardella B., Carlos
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    Autoimmune thyroid disease in the puerperium. Predictive value of thyroid enlargement and related hormonal changes occurring during pregnancy
    (1990) Fardella B., Carlos; Lopez, J. M.; Valdés, M. E.; Nuñez, M.; Miranda, M.
    The incidence of goiter detected during pregnancy and its significance as an indicator of autoimmune thyroid disease after delivery was investigated in a sample of 707 pregnant women (81% in their 2ndtrimester of gestation). Goiter was detected in 106 subjects (15%). Blood T4, T3, TSH, free T4 index (FT4I), antimicrosomal antibodies (AMA) and urinary iodine excretion were measured in these women and in a control group of gravidas without goiter. These measurements were repeated at 1 and 3 months after delivery. Compared with controls during pregnancy, subjects with goiter had lower FT4I values (11.0 2.8 vs 9.0 1.8; p < 0.01) and higher TSH values (2.9 0.6 ?U/ml vs 4.2 2.1 ?U/ml; p < 0.01). In contrast, T4, T3, AMA and urinary iodine excretion values were similar in both groups. In subjects with goiter FT4I values increased over pregnancy levels at 1 month (11.2 2.0; p < 0.05) and 3 months (14.0 3.0; p < 0.05) after delivery; in 29% a biochemical hyperthyroidism (FT4I > 13.5) was detected. During the same period TSH values decreased significantly (1 month: 1.9 0.7 ?U/ml; p < 0.05; 3 months: 2.7 3.0 ?U/ml; p < 0.05). Frequency of positive AMA increased from 8.6% during pregnancy up to 32.1% in the post-delivery period (p < 0.01). In the control group no variation in the FT4I, TSH or AMA were observed after delivery. These results indicate that goiter during pregnancy is common in Chilean gravidas and that it has predictive value for the appearance of autoimmune thyroid disease after delivery.
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    Beneficial effects of mineralocorticoid receptor blockade in experimental non-alcoholic steatohepatitis
    (2015) Pizarro, M.; Solís, Nancy; Quintero, P.; Barrera Martínez, Francisco José; Cabrera, D.; Rojas-de Santiago, P.; Arab Verdugo, Juan Pablo; Padilla, O.; Roa Strauch, Juan Carlos Enrique; Moshage, H.; Wree, A.; Inzaugarat, E.; Feldstein, A.; Fardella B., Carlos; Baudrand Biggs, René; Riquelme, A.; Arrese Jiménez, Marco
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    Clinical, Biochemical, and Genetic Characteristics of "Nonclassic" Apparent Mineralocorticoid Excess Syndrome
    (2019) Tapia Castillo, Alejandra; Baudrand Biggs, René; Vaidya, Anand; Campino Johnson, María del Carmen; Allende, Fidel; Carvajal Maldonado, Cristián Andrés; Vecchiola Cárdenas, Andrea Paola; Lagos Arévalo, Carlos Fernando; Fuentes Zúñiga, Cristóbal Andrés; Fardella B., Carlos; Solari, Sandra; Martínez Aguayo, Alejandro Gregorio; García Bruce, Hernán; Valdivia, Carolina; Tapia Castillo, Alejandra; Baudrand Biggs, René; Vaidya, Anand; Campino Johnson, María del Carmen; Allende, Fidel; Carvajal Maldonado, Cristián Andrés; Vecchiola Cárdenas, Andrea Paola; Lagos Arévalo, Carlos Fernando; Fuentes Zúñiga, Cristóbal Andrés; Fardella B., Carlos; Solari, Sandra; Martínez Aguayo, Alejandro Gregorio; García Bruce, Hernán; Valdivia, Carolina
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    Computed Tomography and Adrenal Venous Sampling in the Diagnosis of Unilateral Primary Aldosteronism
    (2018) Williams, Tracy A.; Burrello, Jacopo; Sechi, Leonardo A.; Fardella B., Carlos; Matrozova, Joanna; Adolf, Christian; Baudrand Biggs, René; Bernardi, Stella; Beuschlein, Felix; Catena, Cristiana; Doumas, Michalis; Fallo, Francesco; Giacchetti, Gilbert
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    Continuum of Renin-Independent Aldosteronism in Normotension
    (2017) Baudrand Biggs, René; Guarda Vega, Francisco; Fardella B., Carlos; Hundemer, G.; Brown, J.; Williams, G.; Vaidya, A.
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    Cortisol/cortisone ratio and matrix metalloproteinase-9 activity are associated with pediatric primary hypertension
    (2016) Martínez Aguayo, Alejandro Gregorio; Campino Johnson, María del Carmen; Baudrand Biggs, René; Carvajal, C.; García Bruce, Hernán; Aglony Imbarack, Marlene Elizabeth; Bancalar, R.; García, L.; Loureiro Pérez, Carolina Andrea; Vecchiola Cárdenas, Andrea Paola; Tapia Castillo, A.; Valdivia, C.; Sanhueza, S.; Fuentes, C.; Lagos, C.; Solari, S.; Allende, Fidel; Kalergis Parra, Alexis Mikes; Fardella B., Carlos; Martínez Aguayo, Alejandro Gregorio; Campino Johnson, María del Carmen; Baudrand Biggs, René; Carvajal, C.; García Bruce, Hernán; Aglony Imbarack, Marlene Elizabeth; Bancalar, R.; García, L.; Loureiro Pérez, Carolina Andrea; Vecchiola Cárdenas, Andrea Paola; Tapia Castillo, A.; Valdivia, C.; Sanhueza, S.; Fuentes, C.; Lagos, C.; Solari, S.; Allende, Fidel; Kalergis Parra, Alexis Mikes; Fardella B., Carlos
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    Depressive symptoms are associated with higher morning plasma cortisol in primary care subjects
    (2018) Capponi, Valentina; Carrasco, Carmen; Macchiavello, Stefano; Undurraga, Juan; Campino Johnson, María del Carmen; Carvajal, Cristian; Gomez, Teresita; Weiss, Cristian; Aedo Campos, Igor Iván; Vecchiola Cárdenas, Andrea Paola; Allende, Fidel; Solari, Sandra; Fardella B., Carlos; Baudrand Biggs, René; Capponi, Valentina; Carrasco, Carmen; Macchiavello, Stefano; Undurraga, Juan; Campino Johnson, María del Carmen; Carvajal, Cristian; Gomez, Teresita; Weiss, Cristian; Aedo Campos, Igor Iván; Vecchiola, Andrea; Allende, Fidel; Solari, Sandra; Fardella B., Carlos; Baudrand Biggs, René
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    Detection of a novel severe mutation affecting the CYP21A2 gene in a Chilean male with salt wasting congenital adrenal hyperplasia
    (2020) Arteaga U., Eugenio; Valenzuela Pino, Felipe Patricio; Lagos, C. F.; Lagos, M.; Martínez García, Alejandra Constanza; Baudrand Biggs, René; Carvajal Maldonado, Cristián Andrés; Fardella B., Carlos
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    Different effects of progesterone and estradiol on chimeric and wild type aldosterone synthase in vitro
    (2013) Vecchiola Cárdenas, Andrea Paola; Lagos Arévalo, Carlos Fernando; Fuentes Zúñiga, Cristóbal Andrés; Allende, Fidel; Campino Johnson, María del Carmen; Valdivia, Carolina.; Tapia Castillo, Alejandra.; Owen, Gareth Ivor; Solari Gajardo, Sandra; Carvajal Maldonado, Cristián Andrés; Fardella B., Carlos; Ogishima, Tadashi.; Mukai, Kuniaki.
    Abstract Background Familial hyperaldosteronism type I (FH-I) is caused by the unequal recombination between the 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes, resulting in the generation of a CYP11B1/B2 chimeric gene and abnormal adrenal aldosterone production. Affected patients usually show severe hypertension and an elevated frequency of stroke at a young age. Aldosterone levels rise during pregnancy, yet in pregnant women with FH-1, their hypertensive condition either remains unchanged or may even improve. The purpose of this study was to investigate in vitro whether female sex steroids modulate the activity of chimeric (ASCE) or wild type (ASWT) aldosterone synthase enzymes. Methods We designed an in vitro assay using HEK-293 cell line transiently transfected with vectors containing the full ASCE or ASWT cDNAs. Progesterone or estradiol effects on AS enzyme activities were evaluated in transfected cells incubated with deoxycorticosterone (DOC) alone or DOC plus increasing doses of these steroids. Results In our in vitro model, both enzymes showed similar apparent kinetic parameters (Km = 1.191 microM and Vmax = 27.08 microM/24 h for ASCE and Km = 1.163 microM and Vmax = 36.98 microM/24 h for ASWT; p = ns, Mann–Whitney test). Progesterone inhibited aldosterone production by ASCE- and ASWT-transfected cells, while estradiol demonstrated no effect. Progesterone acted as a competitive inhibitor for both enzymes. Molecular modelling studies and binding affinity estimations indicate that progesterone might bind to the substrate site in both ASCE and ASWT, supporting the idea that this steroid could regulate these enzymatic activities and contribute to the decay of aldosterone synthase activity in chimeric gene-positive patients. Conclusions Our results show an inhibitory action of progesterone in the aldosterone synthesis by chimeric or wild type aldosterone synthase enzymes. This is a novel regulatory mechanism of progesterone action, which could be involved in protecting pregnant women with FH-1 against hypertension. In vitro, both enzymes showed comparable kinetic parameters, but ASWT was more strongly inhibited than ASCE. This study implicates a new role for progesterone in the regulation of aldosterone levels that could contribute, along with other factors, to the maintenance of an adequate aldosterone-progesterone balance in pregnancy.Abstract Background Familial hyperaldosteronism type I (FH-I) is caused by the unequal recombination between the 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes, resulting in the generation of a CYP11B1/B2 chimeric gene and abnormal adrenal aldosterone production. Affected patients usually show severe hypertension and an elevated frequency of stroke at a young age. Aldosterone levels rise during pregnancy, yet in pregnant women with FH-1, their hypertensive condition either remains unchanged or may even improve. The purpose of this study was to investigate in vitro whether female sex steroids modulate the activity of chimeric (ASCE) or wild type (ASWT) aldosterone synthase enzymes. Methods We designed an in vitro assay using HEK-293 cell line transiently transfected with vectors containing the full ASCE or ASWT cDNAs. Progesterone or estradiol effects on AS enzyme activities were evaluated in transfected cells incubated with deoxycorticosterone (DOC) alone or DOC plus increasing doses of these steroids. Results In our in vitro model, both enzymes showed similar apparent kinetic parameters (Km = 1.191 microM and Vmax = 27.08 microM/24 h for ASCE and Km = 1.163 microM and Vmax = 36.98 microM/24 h for ASWT; p = ns, Mann–Whitney test). Progesterone inhibited aldosterone production by ASCE- and ASWT-transfected cells, while estradiol demonstrated no effect. Progesterone acted as a competitive inhibitor for both enzymes. Molecular modelling studies and binding affinity estimations indicate that progesterone might bind to the substrate site in both ASCE and ASWT, supporting the idea that this steroid could regulate these enzymatic activities and contribute to the decay of aldosterone synthase activity in chimeric gene-positive patients. Conclusions Our results show an inhibitory action of progesterone in the aldosterone synthesis by chimeric or wild type aldosterone synthase enzymes. This is a novel regulatory mechanism of progesterone action, which could be involved in protecting pregnant women with FH-1 against hypertension. In vitro, both enzymes showed comparable kinetic parameters, but ASWT was more strongly inhibited than ASCE. This study implicates a new role for progesterone in the regulation of aldosterone levels that could contribute, along with other factors, to the maintenance of an adequate aldosterone-progesterone balance in pregnancy.
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    Downregulation of exosomal miR-192-5p and miR-204-5p in subjects with nonclassic apparent mineralocorticoid excess.
    (2019) Tapia Castillo, Alejandra.; Barros, Eric.; Allende, Fidel; Vecchiola Cárdenas, Andrea Paola; Fardella B., Carlos; Carvajal Maldonado, Cristián Andrés; Guanzon, Dominic.; Palma, Carlos.; Lai, Andrew.; Salomón Gallo, Carlos Francisco.
    Abstract Background The “nonclassic” apparent mineralocorticoid excess (NC-AME) has been identified in approximately 7% of general population. This phenotype is characterized by low plasma renin activity (PRA), high serum cortisol (F) to cortisone (E) ratio, low cortisone, high Fractional Excretion of potassium (FEK) and normal-elevated systolic blood pressure (SBP). An early detection and/or identification of novel biomarkers of this phenotype could avoid the progression or future complications leading to arterial hypertension. Isolation of extracellular vesicles, such as exosomes, in specific biofluids support the identification of tissue-specific RNA and miRNA, which may be useful as novel biomarkers. Our aim was to identify miRNAs within urinary exosomes associated to the NC-AME phenotype. Methods We perform a cross-sectional study in a primary care cohort of 127 Chilean subjects. We measured BP, serum cortisol, cortisone, aldosterone, PRA. According to the previous reported, a subgroup of subjects was classified as NC-AME (n = 10). Urinary exosomes were isolated and miRNA cargo was sequenced by Illumina-NextSeq-500. Results We found that NC-AME subjects had lower cortisone (p < 0.0001), higher F/E ratio (p < 0.0001), lower serum potassium (p = 0.009) and higher FEK 24 h (p = 0.03) than controls. We found miR-204-5p (fold-change = 0.115; p 0.001) and miR-192-5p (fold-change = 0.246; p 0.03) are both significantly downregulated in NC-AME. miR-192-5p expression was correlated with PRA (r = 0.45; p 0.028) and miR-204-5p expression with SBP (r = − 0.48, p 0.027) and F/E ratio (r = − 0.48; p 0.026). Conclusions These findings could support a potential role of these miRNAs as regulators and novel biomarkers of the NC-AME phenotype.
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    Epigenetics and arterial hypertension : the challenge of emerging evidence
    (2015) Friso, Simonetta; Carvajal Maldonado, Cristián Andrés; Fardella B., Carlos; Olivieri, Oliviero
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    Eplerenone Implantation Improved Adipose Dysfunction Averting RAAS Activation and Cell Division
    (2020) Vecchiola Cárdenas, Andrea Paola; Fuentes, C. A.; Solar, I.; Lagos, C. F.; Opazo, M. C.; Muñoz Durango, Natalia; Riedel Soria, Claudia; Owen, Gareth Ivor; Kalergis Parra, Alexis Mikes; Fardella B., Carlos
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    Esteatosis Hepática: ¿Preludio de diabetes tipo 2 en población pediátrica?
    (2014) Piazzarollo Loureiro, Carolina; Martínez Aguayo, Alejandro Gregorio; Campino Johnson, María del Carmen; Carvajal Maldonado, Cristián Andrés; Fardella B., Carlos; García Bruce, Hernán
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    Excess Iodide Induces an Acute Inhibition of the Sodium/Iodide Symporter in Thyroid Male Rat Cells by Increasing Reactive Oxygen Species
    (2015) Arriagada, A.; Albornoz, E.; Opazo, M.; Becerra, A.; Vidal G.; Fardella B., Carlos; Michea, L.; Carrasco, N.; Simon, F.; Elorza, A.; Bueno Ramírez, Susan; Kalergis Parra, Alexis Mikes; Riedel, C.
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    High sodium intake is associated with increased glucocorticoid production, insulin resistance and metabolic syndrome
    (2014) Baudrand Biggs, René; Campino Johnson, María del Carmen; Carvajal Maldonado, Cristián Andrés; Olivieri, O.; Guidi, G.; Faccini, G.; Vöhringer, P.A.; Cerda, Jaime; Owen, Gareth Ivor; Kalergis Parra, Alexis Mikes; Fardella B., Carlos
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    Hiperaldoteronismo primario y otras formas de hipertension arterial endocrina
    (2016) Carvajal Maldonado, Cristián Andrés; Baudrand Biggs, René; Fardella B., Carlos