Browsing by Author "Contreras Duarte, Susana de las Mercedes"
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- ItemAdenosine receptors: Modulators of lipid availability that are controlled by lipid levels(2017) Leiva Mendoza, Andrea Alejandra; Guzmán Gutiérrez, Enrique; Contreras Duarte, Susana de las Mercedes; Fuenzalida Saavedra, Bárbara; Cantin, Claudette; Carvajal, Lorena; Salsoso Rodríguez, M. Rocío; Gutiérrez, Jaime; Pardo, Fabián; Sobrevía Luarte, Luis Alberto
- ItemDevelopmental abnormalities in mouse embryos lacking the HDL receptor SR-BI(2013) Santander, Nicolás Guillermo; Contreras Duarte, Susana de las Mercedes; Awad, María Fernanda; Lizama, Carlos; Passalacqua, Isabella; Rigotti Rivera, Attilio; Busso, Dolores
- ItemDisfuncionalidad antioxidante de las lipoproteínas de alta densidad (HDL) en pacientes diabéticos descompensados(2015) Awad, Fernanda; Contreras Duarte, Susana de las Mercedes; Molina, Patricia; Quiñones, Verónica; Serrano, V.; Abbott Cáceres, Eduardo Francisco; Maiz, Alberto; Busso, Dolores; Rigotti Rivera, Attilio
- ItemFoetoplacental epigenetic changes associated with maternal metabolic dysfunction(2018) Kerr, Bredford; Leiva Mendoza, Andrea Alejandra; Farías Jofré, Marcelo Enrique; Contreras Duarte, Susana de las Mercedes; Toledo, Fernando; Stolzenbach, Francisca; Silva, Luis; Sobrevía Luarte, Luis Alberto
- ItemGugulipid causes hypercholesterolemia leading to endothelial dysfunction, increased atherosclerosis, and premature death by ischemic heart disease in male mice.(2017) Contreras Duarte, Susana de las Mercedes; Leiva Mendoza, Andrea Alejandra; Amigo, Ludwig; Sepulveda, Esteban; Boric P., Mauricio; Quinones, Veronica; Busso, Dolores; Rigotti Rivera, Attilio
- ItemHigh density lipoprotein cholesterol and proteome in SR-B1 KO mice: lost in precipitation(2018) Contreras Duarte, Susana de las Mercedes; Santander Grez, Nicolás Guillermo; Rigotti Rivera, Attilio; Busso, Dolores; Birner-Gruenberger, Ruth.; Wadsack, Christian.Abstract Scavenger receptor class B type 1 (SR-B1) plays an essential role in high density lipoprotein (HDL) metabolism. SR-B1 deficient (SR-B1 KO) mice are prone to atherosclerosis and exhibit abnormally large, cholesterol-rich, dysfunctional HDL. In a recent issue of J Transl Med, Cao et al. described results of proteomics analyses of HDL isolated from wild-type (WT) and SR-B1 KO mice using precipitation of large lipoproteins with polyethylene glycol (PEG). They report abnormalities in SR-B1 KO HDL protein components that correlate with HDL function. In this commentary, we describe and discuss the differences in the results published by Cao et al. and those obtained in a recent study from our laboratory using shotgun proteomics of HDL of SR-B1 KO mice isolated by ultracentrifugation. We propose that different HDL purification procedures used may account for the discrepancies observed. We show that SR-B1 KO HDL purification using either PEG or dextran sulfate precipitation results in enrichment of small HDL subclasses, and may therefore underestimate alterations in lipoprotein composition or function. Compared to HDL obtained by ultracentrifugation, HDL isolated by PEG precipitation show a lower ApoE/ApoA-I proportion and reduced cholesterol content. HDL protein components described by Cao et al. or our laboratory are mostly inconsistent: only 33 HDL proteins were detected in both datasets, whereas a significant number of proteins were only identified by Cao et al. (n = 43) or Contreras-Duarte et al. (n = 26) datasets. The relative abundance of HDL-associated peptide and protein levels in WT vs SR-B1 HDL were also highly different in both datasets. This study indicates that caution must be taken when interpreting results from HDL isolated by chemical precipitation.
- ItemInterferon-gamma and high glucose-induced opening of Cx43 hemichannels causes endothelial cell dysfunction and damage(2020) Sáez, Juan Carlos; Contreras Duarte, Susana de las Mercedes; Labra, V. C.; Santibañez, C. A.; Mellado, L. A.; Inostroza, C. A.; Alvear, T. F.; Retamal, M. A.; Velarde Aliaga, María Victoria; Orellana, J. A.
- ItemMaternal Dyslipidaemia in Pregnancy with Gestational Diabetes Mellitus: Possible Impact in the Foetoplacental Vascular Function and the Lipoprotein in the Neonatal Circulation.(2017) Contreras Duarte, Susana de las Mercedes; Carvajal, L.; Fuenzalida Saavedra, Bárbara; Cantin, C.; Sobrevía Luarte, Luis Alberto; Leiva Mendoza, Andrea Alejandra
- ItemMaternal supraphysiological hypercholesterolemia associates with endothelial dysfunction of the placental microvasculature(2018) Fuenzalida Saavedra, Bárbara; Sobrevia, Bastián; Cantin, Claudette; Carvajal, Lorena; Salsoso Rodríguez, M. Rocío; Gutiérrez, Jaime; Contreras Duarte, Susana de las Mercedes; Sobrevía Luarte, Luis Alberto; Leiva Mendoza, Andrea Alejandra
- ItemPapel protector de las lipoproteínas de alta densidad en sepsis : aspectos básicos e implicancias clínicas(2014) Contreras Duarte, Susana de las Mercedes; Varas Arancibia, Pablo Andrés; Awad, F.; Busso, Dolores; Rigotti Rivera, Attilio
- ItemRole of extracellular vesicles in glioma progression(2018) Quezada, Claudia; Torres, Ángelo; Niechi, Ignacio; Uribe, Daniel; Contreras Duarte, Susana de las Mercedes; Toledo, Fernando; San Martín, Rody; Gutiérrez Pérez, Jaime Agustín; Sobrevía Luarte, Luis Alberto