Browsing by Author "Casar Leturia, Juan Carlos"
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- ItemAndrographolide attenuates skeletal muscle dystrophy in mdx mice and increases efficiency of cell therapy by reducing fibrosis(2014) Cabrera, D.; Gutiérrez, J.; Cabello Verrugio, Claudio Alejandro; Morales, M. G.; Mezzano, S.; Fadic Ruiz, Ricardo Julio Nicolás; Casar Leturia, Juan Carlos; Hancke, J. L.; Brandan, EnriqueBackground: Duchenne muscular dystrophy (DMD) is characterized by the absence of the cytoskeletal protein dystrophin, muscle wasting, increased transforming growth factor type beta (TGF-β) signaling, and fibrosis. At the present time, the only clinically validated treatments for DMD are glucocorticoids. These drugs prolong muscle strength and ambulation of patients for a short term only and have severe adverse effects. Andrographolide, a bicyclic diterpenoid lactone, has traditionally been used for the treatment of colds, fever, laryngitis, and other infections with no or minimal side effects. We determined whether andrographolide treatment of mdx mice, an animal model for DMD, affects muscle damage, physiology, fibrosis, and efficiency of cell therapy.Methods: mdx mice were treated with andrographolide for three months and skeletal muscle histology, creatine kinase activity, and permeability of muscle fibers were evaluated. Fibrosis and TGF-β signaling were evaluated by indirect immunofluorescence and Western blot analyses. Muscle strength was determined in isolated skeletal muscles and by a running test. Efficiency of cell therapy was determined by grafting isolated skeletal muscle satellite cells onto the tibialis anterior of mdx mice.Results: mdx mice treated with andrographolide exhibited less severe muscular dystrophy than untreated dystrophic mice. They performed better in an exercise endurance test and had improved muscle strength in isolated muscles, reduced skeletal muscle impairment, diminished fibrosis and a significant reduction in TGF-β signaling. Moreover, andrographolide treatment of mdx mice improved grafting efficiency upon intramuscular injection of dystrophin-positive satellite cells.Conclusions: These results suggest that andrographolide could be used to improve quality of life in individuals with DMD.
- ItemHeparan sulfate proteoglycans are increased during skeletal muscle regeneration: requirement of syndecan-3 for successful fiber formation(2004) Casar Leturia, Juan Carlos; Inestrosa Cantín, Nibaldo; Brandan, Enrique
- ItemNon-dystrophic myotonia Chilean cohort with predominance of the SCN4A Gly1306Glu variant(2020) Avila Smirnow, Daniela; Vargas Leal, C. P.; Beytía Reyes, María de Los Ángeles; Cortés Zepeda, R.; Escobar Henríquez, Raúl; Kleinsteuber Saa, K.; Lagos Lucero, Sonia Marcela; Avaria Benapres, M. D.; Padilla Pérez, O.; Casar Leturia, Juan Carlos; Mellado Sagredo, C.; Sternberg, D.
- ItemPesquisa de infección tuberculosa latente en personal de la salud en cuatro instituciones de salud en Santiago de Chile(2014) Hernández, Mariluz; Casar Leturia, Juan Carlos; García Cañete, Patricia; Morales, Verónica; Mamani, Nora; Gómez Cofré, Natalia; Pizarro, Patricia; Balcells Marty, María Elvira
- ItemReduced Natriuresis After Oral Sodium Load in Cholestatic Rats: Role of Compartment Volumes and Anp(2000) Casar Leturia, Juan Carlos; Boric P., Mauricio
- ItemTransient Up-Regulation of Biglycan During Skeletal Muscle Regeneration: Delayed Fiber Growth Along With Decorin Increase in Biglycan-Deficient Mice(2004) Casar Leturia, Juan Carlos; Brandan, Enrique