Browsing by Author "Carrillo, Daniela"

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    Blends based on amino acid functionalized poly (ethylene-alt-maleic anhydride) polyelectrolytes and PEO for nanofiber elaboration: Biocompatible and angiogenic polyelectrolytes
    (2022) Leal, Matias; Leiva, Angel; Villalobos, Valeria; Palma, Veronica; Carrillo, Daniela; Edwards, Natalie; Maine, Arianne; Cauich-Rodriguez, Juan, V; Tamayo, Laura; Neira-Carrillo, Andronico; Urzua, Marcela
    A wide variety of polymers have been electrospun to obtain nanofibers. However, obtaining nanofibers from polyelectrolytes is less frequent due to the charges of these polymers, which hinder the electrospinning process. Poly (ethylene-alt-maleic anhydride) (PEMA) was modified with a series of amino acids (Aa). The functionalization of PEMA with Aa (PEMA-Aa) was demonstrated by FT-IR, H-1 NMR, and C-13 NMR. Blends of PEMA-Aa and poly (ethylene oxide), PEO, with different ratios were prepared. Nanofibers were obtained by electrospinning using blends of 10-20% w/v of PEMA-Aa and 10% w/v of PEO. The conductivity of blends decreased, and the surface tension increased as the quantity of PEO in the blends was increased. TGA showed intermediate thermal properties compared with the blend components. Nanofibers were obtained for all PEMA-Aa/PEO blends, with diameters between 170 and 350 nm. Continuous fibers without morphological defects were obtained at concentrations of 20% w/v and 10% w/v of PEMA-Aa and PEO. Wharton's Jelly Mesenchymal Stem Cells viability, chicken embryo chorioallantoic membrane (CAM) assay and embryo viability measurements were realized for PEMA-Aa. Cytotoxicity test showed both composition and concentration-dependent behavior for PEMA-Aa, with higher WJ-MSC viability at 0.1 mg/mL at 24 h. CAM assay showed the formation of a high number of blood vessels and chicken embryo viability was close to 100% in the presence of polyelectrolytes. This, study demonstrates that electrospun nanofibers obtained from PEMA-Aa/PEO modified polyelectrolyte blends can be considered as a promising material for biomedical applications.
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    Niños y adolescentes con necesidades especiales de atención en salud: prevalencia hospitalaria y riesgos asociados
    (SOC MEDICA SANTIAGO, 2012) Carlos Flores, Juan; Carrillo, Daniela; Karzulovic, Lorena; Cerda, Jaime; Araya, Gabriela; Soledad Matus, Maria; Llevenes, Guillermo; Menchaca, Gonzalo; Vargas, Nelson A.
    Background: "Children with special health care needs" (CSHCN) is a novel definition for pediatric patients with chronic diseases, adopted by the Chilean Pediatric Society in 2008. As life expectancy in Chile increases, prevalence of CSHCN is progressively growing, leading to higher health costs. Aim: To describe the epidemiological profile and clinical characteristics of hospitalized CSHCN, and compare the risk assessment of adverse events during hospitalization using two definitions for CSHCN. Patients and Methods: A cohort of hospitalized CSHCN in a Pediatric Center at Santiago, Chile, was followed from September to December 2009. Clinical and demographic data were registered in a database, including admission to intensive care unit (ICU), nosocomial infections and prolonged hospitalization (> 7 days). Incidence ratios for these events were compared between CSHCN and non-CSHCN, and between children attended by three or more health care professionals (CSHCN-3) and non-CSHCN. Results: Nine hundred twenty patients were included (54% male), with a median age 14 months (0-221) and median days of hospitalization 4 days (1-229). Prevalence of CSHCN was 60.8% of hospitalized children. When using CSHCN definition and comparing with non-CSHCN, no excess of risk was documented. On the other hand, prevalence of CSHCN-3 was 19.9%. Compared to non-CSHCN, these patients had a higher risk of ICU admission (relative risk (RR) 1.58; 95% confidence intervals (CI) = 1.22-2.05; p < 0.01), nosocomial infections (RR 2.28; 95% CI = 1.54-3.39; p < 0.001) and prolonged hospitalization (RR 1.99; 95% CI = 1.52-2.60; p < 0.001). Conclusions: One in five hospitalized children met CSHCN-3 definition. These patients had an increased risk of adverse events during their hospitalization compared to non-CSHCN. (Rev Med Chile 2012; 140: 458-465).