Browsing by Author "Campino Johnson, María del Carmen"

Now showing 1 - 20 of 42
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    A new presentation of the chimeric CYP11B1/CYP11B2 gene with low prevalence of primary aldosteronism and atypical gene segregation pattern
    (Lippincott Williams & Wilkins, 2012) Carvajal Maldonado, Cristian Andrés; Campino Johnson, María del Carmen; Martínez Aguayo, Alejandro Gregorio; Tichauer Calderón, Juan Enrique; Bancalari, Rodrigo; Valdivia, Carolina; Trejo, Pamela; Aglony Imbarack, Marlene Elizabeth; Baudrand Biggs, René Felipe; Lagos Arévalo, Carlos Fernando; Mellado Sagredo, Cecilia Ximena Del Carmen; García Bruce, Hernán Gabriel; Fardella Bello, Carlos Enrique
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    Age-Related Changes in 11 beta-Hydroxysteroid Dehydrogenase Type 2 Activity in Normotensive Subjects
    (2013) Campino Johnson, María del Carmen; Martínez Aguayo, Alejandro Gregorio; Baudrand Biggs, René; Carvajal Maldonado, Cristián Andrés; Aglony Imbarack, Marlene Elizabeth; García Bruce, Hernán; Padilla Pérez, Oslando; Kalergis Parra, Alexis Mikes; Fardella B., Carlos
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    Aldosterona e IL-17 en la génesis de la hipertensión arterial mineralocorticoídea, un estudio ex vivo
    (2016) Vecchiola Cárdenas, Andrea Paola; Cristóbal Fuentes, Z.; Muñoz Durango, Natalia; Tapia Castillo, Alejandra; González Gómez, Luis M.; Baudrand Biggs, René; Carvajal Maldonado, Cristián Andrés; Campino Johnson, María del Carmen; Kalergis Parra, Alexis Mikes; Carlos, F.; Lagos, A.; Fardella B., Carlos; Vecchiola Cárdenas, Andrea Paola; Cristóbal Fuentes, Z.; Muñoz Durango, Natalia; Tapia Castillo, Alejandra; González Gómez, Luis M.; Baudrand Biggs, René; Carvajal Maldonado, Cristián Andrés; Campino Johnson, María del Carmen; Kalergis Parra, Alexis Mikes; Carlos, F.; Lagos, A.; Fardella B., Carlos
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    Aldosterone as a modulator of immunity: implications in the organ damage
    (2011) Herrada, A.; Campino Johnson, María del Carmen; Fardella B., Carlos; Kalergis Parra, Alexis Mikes
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    Bilateral Oophorectomy in a Pregnant Woman: Hormonal Profile From Late Gestation to Post-Partum: Case Report
    (2005) Villaseca Délano, Paulina; Campino Johnson, María del Carmen; Serón Ferré, María; Arteaga U., Eugenio
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    Bioactive Gh-Like Immunologlobulins G in Active Acromegaly: Response to Long-Term Treatment With Bromocriptine
    (1995) Campino Johnson, María del Carmen; López, José Manuel; Serón Ferré, María
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    Bioactivity of Prolactin Isoforms: Lactation and Recovery of Menses in Nursing Women
    (1999) Campino Johnson, María del Carmen; Serón Ferré, María
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    Bone turnover and density in healthy women during breastfeeding and after weaning
    (1996) Lopez, José M; González, G.; Reyes, V.; Campino Johnson, María del Carmen; Díaz, S.
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    Certain Large Forms of Circulating Immunoreactive Human Growth Hormone Are in Fact Inmunoglobulins
    (1990) Campino Johnson, María del Carmen; Serón Ferré, María
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    Clinical, Biochemical, and Genetic Characteristics of "Nonclassic" Apparent Mineralocorticoid Excess Syndrome
    (2019) Tapia Castillo, Alejandra; Baudrand Biggs, René; Vaidya, Anand; Campino Johnson, María del Carmen; Allende, Fidel; Carvajal Maldonado, Cristián Andrés; Vecchiola Cárdenas, Andrea Paola; Lagos Arévalo, Carlos Fernando; Fuentes Zúñiga, Cristóbal Andrés; Fardella B., Carlos; Solari, Sandra; Martínez Aguayo, Alejandro Gregorio; García Bruce, Hernán; Valdivia, Carolina; Tapia Castillo, Alejandra; Baudrand Biggs, René; Vaidya, Anand; Campino Johnson, María del Carmen; Allende, Fidel; Carvajal Maldonado, Cristián Andrés; Vecchiola Cárdenas, Andrea Paola; Lagos Arévalo, Carlos Fernando; Fuentes Zúñiga, Cristóbal Andrés; Fardella B., Carlos; Solari, Sandra; Martínez Aguayo, Alejandro Gregorio; García Bruce, Hernán; Valdivia, Carolina
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    Clock gene expression in adult primate suprachiasmatic nuclei and adrenal: Is the adrenal a peripheral clock responsive to melatonin?
    (2008) Valenzuela, F.; Campino Johnson, María del Carmen; Torrealba, Fernando; Serón Ferré, María; Valenzuela, F.; Campino Johnson, María del Carmen; Torrealba, Fernando; Serón Ferré, María
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    Cortisol/cortisone ratio and matrix metalloproteinase-9 activity are associated with pediatric primary hypertension
    (2016) Martínez Aguayo, Alejandro Gregorio; Campino Johnson, María del Carmen; Baudrand Biggs, René; Carvajal, C.; García Bruce, Hernán; Aglony Imbarack, Marlene Elizabeth; Bancalar, R.; García, L.; Loureiro Pérez, Carolina Andrea; Vecchiola Cárdenas, Andrea Paola; Tapia Castillo, A.; Valdivia, C.; Sanhueza, S.; Fuentes, C.; Lagos, C.; Solari, S.; Allende, Fidel; Kalergis Parra, Alexis Mikes; Fardella B., Carlos; Martínez Aguayo, Alejandro Gregorio; Campino Johnson, María del Carmen; Baudrand Biggs, René; Carvajal, C.; García Bruce, Hernán; Aglony Imbarack, Marlene Elizabeth; Bancalar, R.; García, L.; Loureiro Pérez, Carolina Andrea; Vecchiola Cárdenas, Andrea Paola; Tapia Castillo, A.; Valdivia, C.; Sanhueza, S.; Fuentes, C.; Lagos, C.; Solari, S.; Allende, Fidel; Kalergis Parra, Alexis Mikes; Fardella B., Carlos
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    Depressive symptoms are associated with higher morning plasma cortisol in primary care subjects
    (2018) Capponi, Valentina; Carrasco, Carmen; Macchiavello, Stefano; Undurraga, Juan; Campino Johnson, María del Carmen; Carvajal, Cristian; Gomez, Teresita; Weiss, Cristian; Aedo Campos, Igor Iván; Vecchiola Cárdenas, Andrea Paola; Allende, Fidel; Solari, Sandra; Fardella B., Carlos; Baudrand Biggs, René; Capponi, Valentina; Carrasco, Carmen; Macchiavello, Stefano; Undurraga, Juan; Campino Johnson, María del Carmen; Carvajal, Cristian; Gomez, Teresita; Weiss, Cristian; Aedo Campos, Igor Iván; Vecchiola, Andrea; Allende, Fidel; Solari, Sandra; Fardella B., Carlos; Baudrand Biggs, René
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    Different effects of progesterone and estradiol on chimeric and wild type aldosterone synthase in vitro
    (2013) Vecchiola Cárdenas, Andrea Paola; Lagos Arévalo, Carlos Fernando; Fuentes Zúñiga, Cristóbal Andrés; Allende, Fidel; Campino Johnson, María del Carmen; Valdivia, Carolina.; Tapia Castillo, Alejandra.; Owen, Gareth Ivor; Solari Gajardo, Sandra; Carvajal Maldonado, Cristián Andrés; Fardella B., Carlos; Ogishima, Tadashi.; Mukai, Kuniaki.
    Abstract Background Familial hyperaldosteronism type I (FH-I) is caused by the unequal recombination between the 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes, resulting in the generation of a CYP11B1/B2 chimeric gene and abnormal adrenal aldosterone production. Affected patients usually show severe hypertension and an elevated frequency of stroke at a young age. Aldosterone levels rise during pregnancy, yet in pregnant women with FH-1, their hypertensive condition either remains unchanged or may even improve. The purpose of this study was to investigate in vitro whether female sex steroids modulate the activity of chimeric (ASCE) or wild type (ASWT) aldosterone synthase enzymes. Methods We designed an in vitro assay using HEK-293 cell line transiently transfected with vectors containing the full ASCE or ASWT cDNAs. Progesterone or estradiol effects on AS enzyme activities were evaluated in transfected cells incubated with deoxycorticosterone (DOC) alone or DOC plus increasing doses of these steroids. Results In our in vitro model, both enzymes showed similar apparent kinetic parameters (Km = 1.191 microM and Vmax = 27.08 microM/24 h for ASCE and Km = 1.163 microM and Vmax = 36.98 microM/24 h for ASWT; p = ns, Mann–Whitney test). Progesterone inhibited aldosterone production by ASCE- and ASWT-transfected cells, while estradiol demonstrated no effect. Progesterone acted as a competitive inhibitor for both enzymes. Molecular modelling studies and binding affinity estimations indicate that progesterone might bind to the substrate site in both ASCE and ASWT, supporting the idea that this steroid could regulate these enzymatic activities and contribute to the decay of aldosterone synthase activity in chimeric gene-positive patients. Conclusions Our results show an inhibitory action of progesterone in the aldosterone synthesis by chimeric or wild type aldosterone synthase enzymes. This is a novel regulatory mechanism of progesterone action, which could be involved in protecting pregnant women with FH-1 against hypertension. In vitro, both enzymes showed comparable kinetic parameters, but ASWT was more strongly inhibited than ASCE. This study implicates a new role for progesterone in the regulation of aldosterone levels that could contribute, along with other factors, to the maintenance of an adequate aldosterone-progesterone balance in pregnancy.Abstract Background Familial hyperaldosteronism type I (FH-I) is caused by the unequal recombination between the 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes, resulting in the generation of a CYP11B1/B2 chimeric gene and abnormal adrenal aldosterone production. Affected patients usually show severe hypertension and an elevated frequency of stroke at a young age. Aldosterone levels rise during pregnancy, yet in pregnant women with FH-1, their hypertensive condition either remains unchanged or may even improve. The purpose of this study was to investigate in vitro whether female sex steroids modulate the activity of chimeric (ASCE) or wild type (ASWT) aldosterone synthase enzymes. Methods We designed an in vitro assay using HEK-293 cell line transiently transfected with vectors containing the full ASCE or ASWT cDNAs. Progesterone or estradiol effects on AS enzyme activities were evaluated in transfected cells incubated with deoxycorticosterone (DOC) alone or DOC plus increasing doses of these steroids. Results In our in vitro model, both enzymes showed similar apparent kinetic parameters (Km = 1.191 microM and Vmax = 27.08 microM/24 h for ASCE and Km = 1.163 microM and Vmax = 36.98 microM/24 h for ASWT; p = ns, Mann–Whitney test). Progesterone inhibited aldosterone production by ASCE- and ASWT-transfected cells, while estradiol demonstrated no effect. Progesterone acted as a competitive inhibitor for both enzymes. Molecular modelling studies and binding affinity estimations indicate that progesterone might bind to the substrate site in both ASCE and ASWT, supporting the idea that this steroid could regulate these enzymatic activities and contribute to the decay of aldosterone synthase activity in chimeric gene-positive patients. Conclusions Our results show an inhibitory action of progesterone in the aldosterone synthesis by chimeric or wild type aldosterone synthase enzymes. This is a novel regulatory mechanism of progesterone action, which could be involved in protecting pregnant women with FH-1 against hypertension. In vitro, both enzymes showed comparable kinetic parameters, but ASWT was more strongly inhibited than ASCE. This study implicates a new role for progesterone in the regulation of aldosterone levels that could contribute, along with other factors, to the maintenance of an adequate aldosterone-progesterone balance in pregnancy.
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    Esteatosis Hepática: ¿Preludio de diabetes tipo 2 en población pediátrica?
    (2014) Piazzarollo Loureiro, Carolina; Martínez Aguayo, Alejandro Gregorio; Campino Johnson, María del Carmen; Carvajal Maldonado, Cristián Andrés; Fardella B., Carlos; García Bruce, Hernán
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    Growth Hormone (Gh) Receptor Antibodies With Gh-Like Activity Occur Spontaneously in Acromegaly
    (1992) Campino Johnson, María del Carmen; López, José Manuel; Serón Ferré, María
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    High sodium intake is associated with increased glucocorticoid production, insulin resistance and metabolic syndrome
    (2014) Baudrand Biggs, René; Campino Johnson, María del Carmen; Carvajal Maldonado, Cristián Andrés; Olivieri, O.; Guidi, G.; Faccini, G.; Vöhringer, P.A.; Cerda, Jaime; Owen, Gareth Ivor; Kalergis Parra, Alexis Mikes; Fardella B., Carlos
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    Hipertension arterial mineralocorticoidea
    (2013) Fardella B., Carlos; Carvajal Maldonado, Cristián Andrés; Campino Johnson, María del Carmen; Tapia Castillo, A.; Martínez Aguayo, Alejandro Gregorio; García Bruce, Hernán
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    Hypertensive Patients That Respond to Aldosterone Antagonists May Have a Nonclassical 11β-HSD2 Deficiency.
    (2017) Tapia Castillo, Alejandra; Carvajal Maldonado, Cristián Andrés; Allende, Fidel; Campino Johnson, María del Carmen; Fardella B., Carlos