Browsing by Author "Burgos, Paula I."

Now showing 1 - 13 of 13
  • Thumbnail Image
    Item
    Association of IL4Rsingle-nucleotide polymorphisms with rheumatoid nodules in African Americans with rheumatoid arthritis
    (2010) Burgos, Paula I.; Causey, Zenoria L.; Tamhane, Ashutosh; Kelley, James M.; Brown, Elizabeth E.; Hughes, Laura B.; Danila, Maria I.; van Everdingen, Amalia; Conn, Doyt L.; Jonas, Beth L.
    Abstract Introduction To determine whether IL4R single-nucleotide polymorphisms (SNPs) rs1805010 (I50V) and rs1801275 (Q551R), which have been associated with disease severity in rheumatoid arthritis (RA) patients of European ancestry, relate to the presence of rheumatoid nodules and radiographic erosions in African Americans. Methods Two IL4R SNPs, rs1805010 and rs1801275, were genotyped in 749 patients from the Consortium for Longitudinal Evaluation of African-Americans with Early Rheumatoid Arthritis (CLEAR) registries. End points were rheumatoid nodules defined as present either by physical examination or by chest radiography and radiographic erosions (radiographs of hands/wrists and feet were scored using the modified Sharp/van der Heijde system). Statistical analyses were performed by using logistic regression modeling adjusted for confounding factors. Results Of the 749 patients with RA, 156 (20.8%) had rheumatoid nodules, with a mean age of 47.0 years, 84.6% female gender, and median disease duration of 1.9 years. Of the 461 patients with available radiographic data, 185 (40.1%) had erosions (score >0); their mean age was 46.7 years; 83.3% were women; and median disease duration was 1.5 years. Patients positive for HLA-DRB1 shared epitope (SE) and autoantibodies (rheumatoid factor (RF) or anti-cyclic citrullinated peptide (CCP)) had a higher risk of developing rheumatoid nodules in the presence of the AA and AG alleles of rs1801275 (odds ratio (OR)adj = 8.08 (95% confidence interval (CI): 1.60-40.89), P = 0.01 and ORadj = 2.97 (95% CI, 1.08 to 8.17), P = 0.04, respectively). Likewise, patients positive for the HLA-DRB1 SE and RF alone had a higher risk of developing rheumatoid nodules in presence of the AA and AG alleles of rs1801275 (ORadj = 8.45 (95% CI, 1.57 to 45.44), P = 0.01, and ORadj = 3.57 (95% CI, 1.18 to 10.76), P = 0.02, respectively) and in the presence of AA allele of rs1805010 (ORadj = 4.52 (95% CI, 1.20 to 17.03), P = 0.03). No significant association was found between IL4R and radiographic erosions or disease susceptibility, although our statistical power was limited by relatively small numbers of cases and controls. Conclusions We found that IL4R SNPs, rs1801275 and rs1805010, are associated with rheumatoid nodules in autoantibody-positive African-American RA patients with at least one HLA-DRB1 allele encoding the SE. These findings highlight the need for analysis of genetic factors associated with clinical RA phenotypes in different racial/ethnic populations.
  • Thumbnail Image
    Item
    Chemosensitizing Effect Of Nordihydroguaiaretic Acid (Ndga) And Its Tetra Acetylated Derivative (Ndgata) On Parental And Multiresistant Ta3 Mouse Mammary Adenocarcinoma Cell Lines
    (2008) Ferreira, Jorge; Pavani, Mario; Jana, Fabian; Burgos, Paula I.; Morello, Antonio; Maya, Juan Diego; Faúndez Cáceres, Mario; Lopez, Americo; De Ioannes I., Alfredo E.; Becker C., María Inés
  • Thumbnail Image
    Item
    Factors predictive of thrombotic events in LUMINA, a multi-ethnic cohort of SLE patients (LXXII)
    (OXFORD UNIV PRESS, 2010) Burgos, Paula I.; McGwin, Gerald, Jr.; Reveille, John D.; Vila, Luis M.; Alarcon, Graciela S.
    Method. SLE patients (ACR criteria), age epsilon 16 years, disease duration 5 years at enrolment (T0), African-American, Hispanic (Texan or Puerto Rican) or Caucasian ethnicity, from LUMINA, a longitudinal cohort, were studied. An event was defined as the presence of arterial or venous thrombosis. Time to the first thrombotic event was examined by univariable and multivariable (MV) Cox models adjusting for pertinent baseline clinical and socio-demographic variables.
  • Thumbnail Image
    Item
    First Latin American clinical practice guidelines for the treatment of systemic lupus erythematosus : Latin American Group for the Study of Lupus (GLADEL, Grupo Latino Americano de Estudio del Lupus)-Pan-American League of Associations of Rheumatology (PANLAR)
    (2018) Pons-Estel, Bernardo A.; Bonfa, Eloisa; Soriano, Enrique R.; Cardiel, Mario H.; Izcovich, Ariel; Popoff, Federico; Criniti, Juan M.; Vásquez, Gloria; Burgos, Paula I.; Llanos Muñoz, Carolina
  • No Thumbnail Available
    Item
    Hemicorea como primera manifestación clínica de lupus eritematoso sistémico
    (2017) Armstrong Bruzzone, Macarena; Iruretagoyena B., Mirentxu; Burgos, Paula I.
  • Thumbnail Image
    Item
    Inhibition of angiogenesis by platelets in systemic sclerosis patients
    (2015) Hirigoyen Pérez, Daniela María.; Burgos, Paula I.; Mezzano, Veronica.; Durán, Josefina; Barrientos, Magaly.; Sáez, Claudia G.; Panes Becerra, Olga Teresa; Mezzano, Diego; Iruretagoyena B., Mirentxu
    Abstract Introduction Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by microvascular damage, inflammation, and fibrosis. It has become increasingly evident that platelets, beyond regulating hemostasis, are important in inflammation and innate immunity. Platelets may be an important source of proinflammatory and profibrotic cytokines in the vascular microenvironment. In this study, we sought to assess the contribution of platelet-derived factors in patients with SSc to the angiogenesis of human dermal microvascular endothelial cells (DMVECs) in a tubule formation assay and to characterize the secretion of profibrotic and proinflammatory cytokines in these platelets. Methods We analyzed platelets obtained from 30 patients with SSc and 12 healthy control subjects. Angiogenesis was evaluated in vitro with a DMVEC tubule formation assay on Matrigel and platelet-derived angiogenic factors such as vascular endothelial growth factor (VEGF), 165b isoform (VEGF165b), and cytokine secretion was evaluated. Platelet serotonin content was also determined. Results When DMVECs were incubated with SSc platelet releasates, tubule formation was significantly inhibited (p < 0.01, t test), and higher expression of endothelin-1 in these cells was observed compared with control subjects (p < 0.05, Mann–Whitney U test). In SSc platelet releasates, VEGF165b was significantly higher (p < 0.05, t test), and the VEGF165b/VEGF ratio was increased compared with that of control subjects. Higher secretion of transforming growth factor β (p < 0.01, t test) and CD40L (p < 0.01, t test) was observed compared with control subjects. Also, intraplatelet serotonin levels were lower in platelets obtained from patients with diffuse SSc compared with patients with limited SSc and control subjects (p < 0.05, t test). Conclusions Our findings suggest that antiangiogenic factors such as VEGF165b, together with proinflammatory and profibrotic factors secreted by platelets, can contribute to the progression of peripheral microvascular damage, defective vascular repair, and fibrosis in patients with SSc.Abstract Introduction Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by microvascular damage, inflammation, and fibrosis. It has become increasingly evident that platelets, beyond regulating hemostasis, are important in inflammation and innate immunity. Platelets may be an important source of proinflammatory and profibrotic cytokines in the vascular microenvironment. In this study, we sought to assess the contribution of platelet-derived factors in patients with SSc to the angiogenesis of human dermal microvascular endothelial cells (DMVECs) in a tubule formation assay and to characterize the secretion of profibrotic and proinflammatory cytokines in these platelets. Methods We analyzed platelets obtained from 30 patients with SSc and 12 healthy control subjects. Angiogenesis was evaluated in vitro with a DMVEC tubule formation assay on Matrigel and platelet-derived angiogenic factors such as vascular endothelial growth factor (VEGF), 165b isoform (VEGF165b), and cytokine secretion was evaluated. Platelet serotonin content was also determined. Results When DMVECs were incubated with SSc platelet releasates, tubule formation was significantly inhibited (p < 0.01, t test), and higher expression of endothelin-1 in these cells was observed compared with control subjects (p < 0.05, Mann–Whitney U test). In SSc platelet releasates, VEGF165b was significantly higher (p < 0.05, t test), and the VEGF165b/VEGF ratio was increased compared with that of control subjects. Higher secretion of transforming growth factor β (p < 0.01, t test) and CD40L (p < 0.01, t test) was observed compared with control subjects. Also, intraplatelet serotonin levels were lower in platelets obtained from patients with diffuse SSc compared with patients with limited SSc and control subjects (p < 0.05, t test). Conclusions Our findings suggest that antiangiogenic factors such as VEGF165b, together with proinflammatory and profibrotic factors secreted by platelets, can contribute to the progression of peripheral microvascular damage, defective vascular repair, and fibrosis in patients with SSc.Abstract Introduction Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by microvascular damage, inflammation, and fibrosis. It has become increasingly evident that platelets, beyond regulating hemostasis, are important in inflammation and innate immunity. Platelets may be an important source of proinflammatory and profibrotic cytokines in the vascular microenvironment. In this study, we sought to assess the contribution of platelet-derived factors in patients with SSc to the angiogenesis of human dermal microvascular endothelial cells (DMVECs) in a tubule formation assay and to characterize the secretion of profibrotic and proinflammatory cytokines in these platelets. Methods We analyzed platelets obtained from 30 patients with SSc and 12 healthy control subjects. Angiogenesis was evaluated in vitro with a DMVEC tubule formation assay on Matrigel and platelet-derived angiogenic factors such as vascular endothelial growth factor (VEGF), 165b isoform (VEGF165b), and cytokine secretion was evaluated. Platelet serotonin content was also determined. Results When DMVECs were incubated with SSc platelet releasates, tubule formation was significantly inhibited (p < 0.01, t test), and higher expression of endothelin-1 in these cells was observed compared with control subjects (p < 0.05, Mann–Whitney U test). In SSc platelet releasates, VEGF165b was significantly higher (p < 0.05, t test), and the VEGF165b/VEGF ratio was increased compared with that of control subjects. Higher secretion of transforming growth factor β (p < 0.01, t test) and CD40L (p < 0.01, t test) was observed compared with control subjects. Also, intraplatelet serotonin levels were lower in platelets obtained from patients with diffuse SSc compared with patients with limited SSc and control subjects (p < 0.05, t test). Conclusions Our findings suggest that antiangiogenic factors such as VEGF165b, together with proinflammatory and profibrotic factors secreted by platelets, can contribute to the progression of peripheral microvascular damage, defective vascular repair, and fibrosis in patients with SSc.
  • Thumbnail Image
    Item
    Interplay between the autophagy-lysosomal pathway and the ubiquitin-proteasome system: a target for therapeutic development in alzheimer's disease
    (2018) Bustamante, H.; Gonzalez, A.; Cerda Troncoso, C.; Shaughnessy, Ronan Patrick; Otth, C.; Soza Gajardo, Andrea; Burgos, Paula I.
  • Thumbnail Image
    Item
    Latitude gradient influences the age of onset of rheumatoid arthritis: a worldwide survey
    (2017) Burgos, Paula I.; Ramos Remos, C.; Ramirez Gomez, A.; Brambila Barba, V.; Barajas Ochoa, A.; Castillo Ortiz, J. D.; Adebajo, A. O.; Espinoza, L. R.
  • Thumbnail Image
    Item
    Mestizos with systemic lupus erythematosus develop renal disease early while antimalarials retard its appearance : data from a Latin American cohort
    (2013) Pons-Estel, G.; Alarcón, G.; Burgos, Paula I.; Hachuel, L.; Boggio, G.; Wojdyla, D.; Massardo Vega, Loreto; Iglesias Gamarra, A.
  • Thumbnail Image
    Item
    Radiographic Severity of Rheumatoid Arthritis in African Americans: Results From a Multicenter Observational Study
    (WILEY-LISS, 2010) Bridges, S. Louis, Jr.; Causey, Zenoria L.; Burgos, Paula I.; Huynh, B. Quynh N.; Hughes, Laura B.; Danila, Maria I.; van Everdingen, Amalia; Ledbetter, Stephanie; Conn, Doyt L.; Tamhane, Ashutosh; Westfall, Andrew O.; Jonas, Beth L.; Callahan, Leigh F.; Smith, Edwin A.; Brasington, Richard; Moreland, Larry W.; Alarcon, Graciela S.; van der Heijde, Desiree M.
    Objective. To describe radiographic changes in African Americans with rheumatoid arthritis (RA) from the Consortium for the Longitudinal Evaluations of African Americans with Early Rheumatoid Arthritis (CLEAR) Registry, a multicenter observational study.
  • Thumbnail Image
    Item
    Smoking promotes exacerbated inflammatory features in dendritic cells of Chilean rheumatoid arthritis patients
    (2018) Prado, Carolina; Iruretagoyena B., Mirentxu; Burgos, Paula I.; Pacheco, Rodrigo
  • Thumbnail Image
    Item
    The prevalence of rheumatoid arthritis in Chile : a nationwide study performed as part of the national health survey
    (2020) Durán, Josefina; Massardo Vega, Loreto; Llanos Muñoz, Carolina; Iacobelli Gabrielli, Sergio Hernán; Burgos, Paula I.; Cisternas Martínez, Marcela Carolina; Iruretagoyena B., Mirentxu; Armstrong Bruzzone, Macarena; Aguilera Insunza, Raquel; Radrigán Araya, Francisco José Ricardo; Martínez Ruiz-Esquide, María Eugenia; Passi Solar, Alvaro Rodrigo; Vásquez Aravena, Nancy Margarita; Margozzini Maira, Paula; Riedemann, P.; Crisóstomo, N.; Cifuentes, C.; Hagedorn, L.; Cisternas, A.
  • Thumbnail Image
    Item
    Understanding the Cryoglobulinemias
    (2019) Fuentes, A.; Mardones, C.; Burgos, Paula I.