Browsing by Author "Bravo Castillo, María Loreto"
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- Item2-Methoxyestradiol inhibits progesterone-dependent tissue factor expression and activity in breast cancer cells(2010) Quezada, M.; Diaz, J.; Henriquez, S.; Bravo Castillo, María Loreto; Aranda, E.; Oliva, B.; Villalón, Manuel J.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Brosens, J. J.; Lange, C. A.; Owen, Gareth Ivor2-Methoxyestradiol (2ME) is an endogenous metabolite of 17β-estradiol with antiangiogenic and antitumor properties, although its mechanisms of action remain unclear. Progestins in hormone replacement therapy increase the risk of breast cancer. Progesteron
- ItemDiabetic concentrations of metformin inhibit platelet-mediated ovarian cancer cell progression(2017) Erices, R.; Cubillos, Sofía; Aravena, Raúl; Santoro, Felice; Márquez, Mónica; Orellana Walden, Renán Felipe; Ramírez, Carolina; González, Pamela; Fuenzalida, Patricia; Bravo Castillo, María Loreto; Oliva, Bárbara; Kato Cardemil, Sumie Rode; Ibáñez, Carolina; Brañes, Jorge; Bravo, Erasmo; Alonso, Catalina
- ItemPaclitaxel-PHBV nanoparticles and their toxicity to endometrial and primary ovarian cancer cells(2013) Vilos, C.; Morales, F.; Solar, P.; Herrera, N.; González Nilo, F.; Aguayo, D.; Mendoza, H.; Bravo Castillo, María Loreto; González, P.; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Owen, Gareth Ivor
- ItemPatient inflammatory status and CD4+/CD8+ intraepithelial tumor lymphocyte infiltration are predictors of outcomes in high-grade serous ovarian cancer(2018) Pinto, Mauricio P.; Balmaceda, Carlos; Bravo Castillo, María Loreto; Kato Cardemil, Sumie Rode; Villarroel, Alejandra; Owen, Gareth Ivor; Roa Strauch, Juan Carlos Enrique; Cuello F., Mauricio; Ibañez, Carolina
- ItemPlatelets enhance tissue factor protein and metastasis initiating cell markers, and act as chemoattractants increasing the migration of ovarian cancer cells.(2015) Orellana, Renan.; Kato Cardemil, Sumie Rode; Erices, Rafaela.; Bravo Castillo, María Loreto; González Hevia, Pamela Andrea.; Oliva, Bárbara.; Cubillos Alfaro, Sofía María Jesús.; Valdivia Román, Andrés Felipe; Brañes, Jorge; Cuello F., Mauricio; Ibañez, Carolina; Barriga Cosmelli, María Isabel; Bravo, Erasmo.; Alonso, Catalina.; Bustamente, Eva.; Castellon, Enrique.; Hidalgo, Patricia.; Trigo, Cesar.; Panes Becerra, Olga Teresa; Pereira Garcés, Jaime Ignacio; Mezzano, Diego; Owen, Gareth IvorAbstract Background An increase in circulating platelets, or thrombocytosis, is recognized as an independent risk factor of bad prognosis and metastasis in patients with ovarian cancer; however the complex role of platelets in tumor progression has not been fully elucidated. Platelet activation has been associated with an epithelial to mesenchymal transition (EMT), while Tissue Factor (TF) protein expression by cancer cells has been shown to correlate with hypercoagulable state and metastasis. The aim of this work was to determine the effect of platelet-cancer cell interaction on TF and “Metastasis Initiating Cell (MIC)” marker levels and migration in ovarian cancer cell lines and cancer cells isolated from the ascetic fluid of ovarian cancer patients. Methods With informed patient consent, ascitic fluid isolated ovarian cancer cells, cell lines and ovarian cancer spheres were co-cultivated with human platelets. TF, EMT and stem cell marker levels were determined by Western blotting, flow cytometry and RT-PCR. Cancer cell migration was determined by Boyden chambers and the scratch assay. Results The co-culture of patient-derived ovarian cancer cells with platelets causes: 1) a phenotypic change in cancer cells, 2) chemoattraction and cancer cell migration, 3) induced MIC markers (EMT/stemness), 3) increased sphere formation and 4) increased TF protein levels and activity. Conclusions We present the first evidence that platelets act as chemoattractants to cancer cells. Furthermore, platelets promote the formation of ovarian cancer spheres that express MIC markers and the metastatic protein TF. Our results suggest that platelet-cancer cell interaction plays a role in the formation of metastatic foci.
- ItemProgesterone regulation of tissue factor depends on MEK1/2 activation and requires the proline-rich site on progesterone receptor(2015) Bravo Castillo, María Loreto; Pinto, Mauricio P.; González, Ibeth; Oliva, Bárbara; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Lange, Carol A.; Owen, Gareth Ivor
- ItemTécnica para cultivar y expandir células de cáncer extraídas de fluido ascitico y su exposición a concentraciones específicas de regímenes de quimioterapia (Chile, concesión n° 55061)Owen, Gareth Ivor; Bravo Castillo, María Loreto; González, Pamela; Kato Cardemil, Sumie Rode; Cuello F., Mauricio; Orellana Walden, Renán Felipe
- ItemTissue factor is regulated by epidermal growth factor in normal and malignant human endometrial epithelial cells(2005) Kato Cardemil, Sumie Rode; Pinto Paganini, Mauricio Arturo; Carvajal, Andrés.; Espinoza, Natalia.; Monsó, Carolina.; Bravo Castillo, María Loreto; Villalón, Manuel J.; Cuello F., Mauricio; Owen, Gareth Ivor