Browsing by Author "Andrés Coke, María Estela"
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- ItemActivation of GABA-B receptors induced by systemic amphetamine abolishes dopamine release in the rat lateral septum(2010) Sotomayor Zárate, Ramón Eduardo; Andrés Coke, María Estela; Gysling Caselli, Katia
- ItemAn Amphipathic Alpha-Helix in the Prodomain of Cocaine and Amphetamine Regulated Transcript Peptide Precursor Serves as Its Sorting Signal to the Regulated Secretory Pathway(2013) Blanco Nahuelqueo, Elías; Lagos Arévalo, Carlos Fernando; Andrés Coke, María Estela; Gysling Caselli, Katia
- ItemAn Early Disturbance in Serotonergic Neurotransmission Contributes to the Onset of Parkinsonian Phenotypes in Drosophila melanogaster(MDPI, 2022) Zárate Canales, Rafaella Victoria; Hidalgo, Sergio; Navarro, Nicole; Molina Mateo, Daniela Francisca; Arancibia, Duxan; Rojo Cortés, Francisca Rayén; Oliva, Carlos; Andrés Coke, María Estela; Zamorano, Pedro; Campusano Astorga, Jorge MauricioParkinson's disease (PD) is a neurodegenerative disease characterized by motor symptoms and dopaminergic cell loss. A pre-symptomatic phase characterized by non-motor symptoms precedes the onset of motor alterations. Two recent PET studies in human carriers of mutations associated with familial PD demonstrate an early serotonergic commitment-alteration in SERT binding-before any dopaminergic or motor dysfunction, that is, at putative PD pre-symptomatic stages. These findings support the hypothesis that early alterations in the serotonergic system could contribute to the progression of PD, an idea difficult to be tested in humans. Here, we study some components of the serotonergic system during the pre-symptomatic phase in a well-characterized Drosophila PD model, Pink1(B9) mutant flies. We detected lower brain serotonin content in Pink1(B9) flies, accompanied by reduced activity of SERT before the onset of motor dysfunctions. We also explored the consequences of a brief early manipulation of the serotonergic system in the development of motor symptoms later in aged animals. Feeding young Pink1(B9) flies with fluoxetine, a SERT blocker, prevents the loss of dopaminergic neurons and ameliorates motor impairment observed in aged mutant flies. Surprisingly, the same pharmacological manipulation in young control flies results in aged animals exhibiting a PD-like phenotype. Our findings support that an early dysfunction in the serotonergic system precedes and contributes to the onset of the Parkinsonian phenotype in Drosophila.
- ItemCharacterizing HSF1 Binding and Post-Translational Modifications of hsp70 Promoter in Cultured Cortical Neurons : Implications in the Heat-Shock Response(2015) Gómez Zúñiga, Andrea Verónica; Córdova, G.; Munita Morgan, Roberto Andrés; Parada, G.; Barrios, Á.; Cancino, G.; Álvarez Rojas, Alejandra; Andrés Coke, María Estela
- ItemCoREST represses the heat shock response mediated by HSF1(2008) Gómez Zúñiga, Andrea Verónica; Galleguillos Caro, Danny; Andrés Coke, María Estela
- ItemCRF binding protein facilitates the presence of CRF type 2α receptor on the cell surface(2016) Slater Guzmán, Paula Gabriela; Cerda, Cledi A.; Pereira, Luis A.; Andrés Coke, María Estela; Gysling Caselli, Katia
- ItemCross-talk between dopamine D1 and corticotropin releasing factor type 2 receptors leads to occlusion of their ERK1/2 signaling(2020) Yarur, H. E.; González Bustos, Marcela Paz; Verbel Vergara, Daniel Eduardo; Andrés Coke, María Estela; Gysling Caselli, Katia
- ItemDecreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation(2015) Sáez, Juan Carlos; Gomez, A.; Barrios, A.; Parada, G.; Galdames, L.; Gonzalez, M.; Andrés Coke, María Estela
- ItemDevelopment of a Bicistronic Vector for the Expression of a CRISPR/Cas9-mCherry System in Fish Cell Lines(2019) Escobar Aguirre, Sebastián Gonzalo; Arancibia Radich, Duxan Andrés; Escorza, A.; Bravo, C.; Andrés Coke, María Estela; Zamorano, P.; Martinez, V.
- ItemDifferential properties of transcriptional complexes formed by the CoREST family(2014) Barrios Saavedra, Alvaro Patricio; Sáez Ardura, Julián Esteban; Andrés Coke, María Estela
- ItemEstrogen Receptors Alpha and Beta Differentially Regulate the Transcriptional Activity of the Urocortin Gene(2006) Haeger, Paola; Andrés Coke, María Estela; Forray Claps, María Inés; Gysling Caselli, Katia
- ItemFunctional and molecular interaction between D2 and kappa opioid receptors: role in locomotor sensitization to quinpirole(2012) Escobar, A.P.; Cornejo Castillo, Francisca Alejandra; Fuentealba Evans, José Antonio; Andrés Coke, María Estela
- ItemHaloperidol-induced Nur77 expression in striatopallidal neurons is under the control of protein phosphatase 1 regulation by DARPP-32(2014) Sánchez Otayza, Natalia Alejandra; Andrés Coke, María Estela
- ItemIncreased locomotor response to amphetamine induced by the repeated administration of the selective kappa-opioid receptor agonist U-69593(2007) Fuentealba Evans, José Antonio; Gysling Caselli, Katia; Andrés Coke, María Estela
- ItemInhibitory Control of Basolateral Amygdalar Transmission to the Prefrontal Cortex by Local Corticotrophin Type 2 Receptor(2020) Yarur, H. E.; Vega Quiroga, I.; González, Marcela; Noches Gallardo, Verónica Andrea; Thomases, D. R.; Andrés Coke, María Estela; Ciruela, F.; Tseng, K. Y.; Gysling Caselli, Katia
- ItemIntron Retention As An Alternative Splice Variant of the Rat Urocortin 1 Gene(2006) Blanco, E.; Andrés Coke, María Estela; Forray Claps, María Inés; Gysling Caselli, Katia
- ItemLong 3'UTR of Nurr1 mRNAs is targeted by miRNAs in mesencephalic dopamine neurons(2017) Pereira, L.; Munita, R.; González Bustos, Marcela Paz; Andrés Coke, María Estela
- ItemLong-term loss of dopamine release mediated by CRF-1 receptors in the rat lateral septum after repeated cocaine administration(2013) Sotomayor Zárate, Ramón Eduardo; Renard, Georgina María; Araya, Katherine A.; Carreño, Paz; Fuentealba Evans, José Antonio; Andrés Coke, María Estela; Gysling Caselli, Katia
- ItemMechanisms of Kappa Opioid Receptor Potentiation of Dopamine D2 Receptor Function in Quinpirole-Induced Locomotor Sensitization in Rats(2017) Escobar Maldonado, Angélica del Pilar; González Bustos, Marcela Paz; Meza, R.; Noches, V.; Henny V., Pablo; Gysling Caselli, Katia; Espana, R.; Fuentealba Evans, José Antonio; Andrés Coke, María Estela
- ItemMicroExonator enables systematic discovery and quantification of microexons across mouse embryonic development(2021) Parada González, Guillermo Eduardo; Munita Robert, Roberto Andrés; Georgakopoulos-Soares, Ilias; Fernandes, Hugo J. R.; Kedlian, Veronika R.; Metzakopian, Emmanouil; Andrés Coke, María Estela; Miska, Eric A.; Hemberg, MartinAbstract Background Microexons, exons that are ≤ 30 nucleotides, are a highly conserved and dynamically regulated set of cassette exons. They have key roles in nervous system development and function, as evidenced by recent results demonstrating the impact of microexons on behaviour and cognition. However, microexons are often overlooked due to the difficulty of detecting them using standard RNA-seq aligners. Results Here, we present MicroExonator, a novel pipeline for reproducible de novo discovery and quantification of microexons. We process 289 RNA-seq datasets from eighteen mouse tissues corresponding to nine embryonic and postnatal stages, providing the most comprehensive survey of microexons available for mice. We detect 2984 microexons, 332 of which are differentially spliced throughout mouse embryonic brain development, including 29 that are not present in mouse transcript annotation databases. Unsupervised clustering of microexons based on their inclusion patterns segregates brain tissues by developmental time, and further analysis suggests a key function for microexons in axon growth and synapse formation. Finally, we analyse single-cell RNA-seq data from the mouse visual cortex, and for the first time, we report differential inclusion between neuronal subpopulations, suggesting that some microexons could be cell type-specific. Conclusions MicroExonator facilitates the investigation of microexons in transcriptome studies, particularly when analysing large volumes of data. As a proof of principle, we use MicroExonator to analyse a large collection of both mouse bulk and single-cell RNA-seq datasets. The analyses enabled the discovery of previously uncharacterized microexons, and our study provides a comprehensive microexon inclusion catalogue during mouse development.
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