Browsing by Author "Amigo Boker, Ludwig Peter"
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- ItemEl ácido nicotínico aumenta el transporte celular de colesterol de las lipoproteínas de alta densidad en pacientes con hipoalfalipoproteinemia(2015) Figueroa, Catalina; Droppelmann Droppelmann, Katherine Ann; Quinones, Verónica; Amigo Boker, Ludwig Peter; Mendoza, Camila; Serrano Larrea, Valentina; Véjar, Margarita; Maiz Gurruchaga, Manuel Alberto; Rigotti Rivera, Attilio
- ItemApolipoprotein A-I deficiency does not affect biliary lipid secretion and gallstone formation in mice(2011) Amigo Boker, Ludwig Peter; Quiñones, Verónica; Leiva Mendoza, Andrea Alejandra; Busso, Dolores; Zanlungo Matsuhiro, Silvana; Nervi, Flavio; Rigotti Rivera, Attilio
- ItemEarly Onset Intrauterine Growth Restriction in a Mouse Model of Gestational Hypercholesterolemia and Atherosclerosis(2014) Busso, Dolores; Mascareno, L.; Salas, F.; Berkowitz Fiebich, Loni; Santander, N.; Quiroz Vallverdu, Alonso Ingmar; Amigo Boker, Ludwig Peter; Valdés Stromilli, Gloria; Rigotti Rivera, Attilio
- ItemFecal bile acid excretion and messenger RNA expression levels of ileal transporters in high risk gallstone patients(2009) Herrera Sepúlveda, Jorge Gabriel; Amigo Boker, Ludwig Peter; Benítez, Carlos; Zanlungo Matsuhiro, Silvana; Miquel P., Juan Francisco; Nervi, Flavio; Husche, Constanze; Lütjohann, DieterAbstract Background Cholesterol gallstone disease (GS) is highly prevalent among Hispanics and American Indians. In GS, the pool of bile acids (BA) is decreased, suggesting that BA absorption is impaired. In Caucasian GS patients, mRNA levels for ileal BA transporters are decreased. We aimed to determine fecal BA excretion rates, mRNA levels for ileal BA transporter genes and of regulatory genes of BA synthesis in Hispanic GS patients. Results Excretion of fecal BA was measured in seven GS females and in ten GS-free individuals, all with a body mass index < 29. Participants ingested the stool marker Cr2O3 (300 mg/day) for 10 days, and fecal specimens were collected on the last 3 days. Chromium was measured by a colorimetric method, and BA was quantitated by gas chromatography/mass spectroscopy. Intake of calories, nutrients, fiber and cholesterol were similar in the GS and GS-free subjects. Mean BA excretion levels were 520 ± 80 mg/day for the GS-free group, and 461 ± 105 mg/day for the GS group. Messenger RNA expression levels were determined by RT-PCR on biopsy samples obtained from ileum during diagnostic colonoscopy (14 GS-free controls and 16 GS patients) and from liver during surgery performed at 8 and 10 AM (12 GS and 10 GS-free patients operated on for gastrointestinal malignancies), all with a body mass index < 29. Messenger RNA level of the BA transporter genes for ileal lipid binding protein, multidrug resistance-associated protein 3, organic solute transporter alpha, and organic solute transporter beta were similar in GS and GS-free subjects. Messenger RNA level of Cyp27A1, encoding the enzyme 27α-hydroxylase, the short heterodimer partner and farnesoid X receptor remained unchanged, whereas the mRNA level of Cyp7A1, the rate limiting step of BA synthesis, was increased more than 400% (p < 0.01) in the liver of GS compared to GS-free subjects. Conclusion Hispanics with GS have fecal BA excretion rates and mRNA levels of genes for ileal BA transporters that are similar to GS-free subjects. However, mRNA expression levels of Cyp7A1 are increased in GS, indicating that regulation of BA synthesis is abnormal in Hispanics with GS.
- ItemImpaired biliary cholesterol secretion and decreased gallstone formation in apolipoprotein E-deficient mice fed a high-cholesterol diet(2000) Amigo Boker, Ludwig Peter; Quiñones, Verónica; Mardones, Pablo; Zanlungo Matsuhiro, Silvana; Miquel Poblete, Juan Francisco; Nervi Oddone, Flavio; Rigotti Rivera, Attilio GianpietroBackground & aimsBecause apolipoprotein E (apoE) is a key cholesterol transport molecule involved in the hepatic uptake of chylomicron cholesterol, it may play a critical role in controlling bile cholesterol elimination and cholesterol gallstone formation induced by dietary cholesterol. To test this hypothesis, we studied biliary lipid secretion and gallstone formation in apoE-deficient mice fed cholesterol-rich diets.MethodsBile lipid outputs and gallstone sequence events were analyzed in apoE-deficient mice fed a high-cholesterol diet or a lithogenic diet compared with control animals.ResultsA high-cholesterol diet increased biliary cholesterol secretion and gallbladder bile cholesterol concentration in wild-type mice; the increase in bile cholesterol secretion was significantly attenuated in apoE-deficient mice. ApoE knockout mice fed a high-cholesterol lithogenic diet had a markedly lower frequency of gallbladder bile cholesterol crystal and gallstone formation than wild-type mice, which was most likely a result of the decreased cholesterol saturation index found in gallbladder bile of apoE-deficient mice.ConclusionsThese results show that apoE expression is an important factor for regulating both biliary secretion of diet-derived cholesterol as well as diet-induced cholesterol gallstone formation in mice.
- ItemMetabolic Effects of Cholecystectomy : Gallbladder Ablation Increases Basal Metabolic Rate through G-Protein Coupled Bile Acid Receptor Gpbar1-Dependent Mechanisms in Mice(2015) Cortés Mora, Víctor Antonio; Amigo Boker, Ludwig Peter; Zanlungo Matsuhiro, Silvana; Galgani Fuentes, José; Robledo, Fermín; Arrese Jiménez, Marco; Bozinovic Kuscevic, Francisco; Nervi, Flavio