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Browsing ANID by Author "Aravena, Gabriela"

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    High prevalence of lupin allergy among patients with peanut allergy: identification of γ-conglutin as majo allergen
    (American College of Allergy, Asthma and Immunology, 2022) Aguilera-Insunza, Raquel; Iturriaga, Carolina; Mariñanco, Angélica; Venegas, Luis; Aravena, Gabriela; Pérez-Mateluna, Guillermo; Baptista-Dias, Nathalia; Borzutzky Schachter, Arturo; Wandersleben, Traudy
    Background: Lupin is a protein-rich legume with a growing presence in the food market worldwide. With increased consumption, lupin allergy (LA) reports are also rising. Uncertainties exist on the cross-reactivity between peanut and lupin, the allergenic potential of different lupin species, and sensitization patterns among different populations. Objective: To evaluate the molecular basis of LA and to determine lupin allergens from 3 different species that may be involved in peanut allergy (PA) cross-reactivity. Methods: A total of 43 subjects with PA, those with LA, or controls without food allergy were evaluated with skin prick tests (SPTs) and specific IgEs (sIgEs). Lupin-sensitized subjects were offered a lupin oral food challenge (OFC). Immunoblots and enzyme-linked immunosorbent assays were performed on sera from lupin-sensitized subjects. Results: In this study, 44% of the PA subjects were confirmed to have LA by OFC. Anaphylaxis was the most frequent manifestation after lupin consumption, with a minimal eliciting dosage of 1 g lupin flour. There was no difference in lupin sIgE or SPT wheal size between lupin-sensitized and confirmed LA subjects or in the severity of symptoms among confirmed LA subjects. Sera from lupin-sensitized subjects uniformly reacted to all 3 different lupin species. Immunoblotting and enzyme-linked immunosorbent assays revealed immunoglobulin E binding to a- and g-conglutin in all analyzed sera, whereas a- and b-conglutin recognition was variable. Conclusion: Our findings reveal a high prevalence of LA among PA subjects, emphasizing lupin must be labeled as an allergen in foods. Owing to high variability in lupin-sIgE and lupin-SPT results, LA diagnosis may require OFC. In our population, g-conglutin is the major allergen of lupin