Involvement of MT1-MMP and TIMP-2 in human periodontal disease
link https://doi.org/10.1111/j.1601-0825.2009.01651.xaccount_box Oyarzun, A.account_box Arancibia, R.account_box Hidalgo, R.account_box Penafiel, C.account_box Caceres, M.account_box Gonzalez, M Jaccount_box Martinez, J.account_box Smith, P. C.
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| Autor | Oyarzun, A. Arancibia, R. Hidalgo, R. Penafiel, C. Caceres, M. Gonzalez, M J Martinez, J. Smith, P. C. |
| Título | Involvement of MT1-MMP and TIMP-2 in human periodontal disease |
| Revista | ORAL DISEASES |
| ISSN | 1354-523X |
| ISSN electrónico | 1601-0825 |
| Volumen | 16 |
| Número de publicación | 4 |
| Página inicio | 388 |
| Página final | 395 |
| Fecha de publicación | 2010 |
| Resumen | Objectives: Periodontal disease is characterized by an increased collagen metabolism. Although membrane type-1 matrix metalloproteinase (MT1-MMP) plays a critical role in collagen degradation, its involvement in human periodontitis remains to be determined. Methods: MT1-MMP and TIMP-2 expression and distribution were evaluated in gingival tissue samples derived from 10 healthy and 12 periodontitis-affected human subjects. MT1-MMP and TIMP-2 expression were assessed through Western-blot of tissue homogenates. The main cell types involved in MT1-MMP and TIMP-2 production were evaluated by means of immunohistochemistry. Results: Both MT1-MMP and TIMP-2 were significantly increased in periodontitis-affected gingival tissues when compared to healthy gingiva. Moreover, the balance between MT1-MMP and its inhibitor TIMP-2 was altered in periodontitis-affected tissues, suggesting an imbalance in this proteolytic axis. Immunohistochemistry demonstrated the expression of MT1-MMP in fibroblasts and macrophages in gingival tissues. MT1-MMP was detected in cells in close association with the gingival collagen matrix. TIMP-2 expression was identified in fibroblasts, macrophages and epithelial cells. Conclusions: Our observations show an increased expression of MT1-MMP and TIMP-2 in periodontitis-affected gingival tissues. The altered balance between these two molecular mediators of collagen remodeling suggests their involvement in human periodontal disease. |
| Derechos | acceso restringido |
| Agencia financiadora | National Fund for Science and Technology from Chile |
| DOI | 10.1111/j.1601-0825.2009.01651.x |
| Editorial | WILEY |
| Enlace | |
| Id de publicación en Pubmed | MEDLINE:20233321 |
| Id de publicación en WoS | WOS:000276247800011 |
| Paginación | 8 páginas |
| Palabra clave | MMP MMP-14 MT1-MMP TIMP-2 periodontal disease TYPE-1 MATRIX-METALLOPROTEINASE GINGIVAL CREVICULAR FLUID NECROSIS-FACTOR-ALPHA 1-MATRIX METALLOPROTEINASE TISSUE INHIBITOR IN-VIVO EXPRESSION COLLAGENASE ACTIVATION SURFACE |
| Tema ODS | 03 Good Health and Well-being |
| Tema ODS español | 03 Salud y bienestar |
| Tipo de documento | artículo |