Microbial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods
link https://doi.org/10.1515/JPM.2010.078account_box DiGiulio, Daniel B.account_box Gervasi, MariaTeresaaccount_box Romero, Robertoaccount_box Mazaki Tovi, Shaliaccount_box Vaisbuch, Ediaccount_box Kusanovic, Juan Pedroaccount_box Seok, Kimberley S.account_box Gomez, Ricardoaccount_box Mittal, Poojaaccount_box Gotsch, Francescaaccount_box Chaiworapongsa, Tinnakornaccount_box Oyarzun, Enriqueaccount_box Kim, Chong Jaiaccount_box Relman, David A.
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Objective: Infection has been implicated in the pathogenesis of preeclampsia, yet the association between microbial invasion of the amniotic cavity (MIAC) and preeclampsia has not been determined. The aim of this study was to determine the prevalence, and microbial diversity associated with MIAC, as well as the nature of the host response to MIAC in patients with preeclampsia.
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| Autor | DiGiulio, Daniel B. Gervasi, MariaTeresa Romero, Roberto Mazaki Tovi, Shali Vaisbuch, Edi Kusanovic, Juan Pedro Seok, Kimberley S. Gomez, Ricardo Mittal, Pooja Gotsch, Francesca Chaiworapongsa, Tinnakorn Oyarzun, Enrique Kim, Chong Jai Relman, David A. |
| Título | Microbial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods |
| Revista | JOURNAL OF PERINATAL MEDICINE |
| ISSN | 0300-5577 |
| ISSN electrónico | 1619-3997 |
| Volumen | 38 |
| Número de publicación | 5 |
| Página inicio | 503 |
| Página final | 513 |
| Fecha de publicación | 2010 |
| Resumen | Objective: Infection has been implicated in the pathogenesis of preeclampsia, yet the association between microbial invasion of the amniotic cavity (MIAC) and preeclampsia has not been determined. The aim of this study was to determine the prevalence, and microbial diversity associated with MIAC, as well as the nature of the host response to MIAC in patients with preeclampsia. Method of study: Amniotic fluid (AF) from 62 subjects with preeclampsia, not in labor, was analyzed with both cultivation and molecular methods. Broad-range and group-specific PCR assays targeting small subunit ribosomal DNA, or other gene sequences, from bacteria, fungi and archaea were used. Results were correlated with measurements of host inflammatory response, including AF white blood cell count and AF concentrations of glucose, interleukin-6 (IL-6) and MMP-8. Results: 1) The rate of MIAC in preeclampsia was 1.6% (1/62) based on cultivation techniques, 8% (5/62) based on PCR, and 9.6% (6/62) based on the combined results of both methods; 2) among the six patients diagnosed with MIAC, three had a positive PCR for Sneathia/Leptotrichia spp.; and 3) patients with MIAC were more likely to have evidence of an inflammatory response in the amniotic cavity than those without MIAC, as determined by a higher median AF IL-6 [1.65 ng/mL interquartile range (IQR): 0.35-4.62 vs. 0.22 ng/mL IQR: 0.12-0.51; P=0.002). Conclusion: The prevalence of MIAC in preeclampsia is low, suggesting that intra-amniotic infection plays only a limited role in preeclampsia. However, the unexpectedly high number of positive AF specimens for Sneathia/Leptotrichia warrants further investigation. |
| Derechos | registro bibliográfico |
| Agencia financiadora | Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, DHHS March of Dimes Foundation NIH Thomas C. and Joan M. Merigan Endowment at Stanford University EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH |
| DOI | 10.1515/JPM.2010.078 |
| Editorial | WALTER DE GRUYTER GMBH |
| Enlace | |
| Id de publicación en Pubmed | MEDLINE:20482470 |
| Id de publicación en WoS | WOS:000281566600009 |
| Paginación | 11 páginas |
| Palabra clave | IL-6 intra-amniotic infection intra-amniotic inflammation PCR preeclampsia pregnancy 16S rRNA Sneathia/Leptotrichia spp. Ureaplasma urealyticum ENDOTHELIAL GROWTH-FACTOR FOR-GESTATIONAL-AGE TUMOR-NECROSIS-FACTOR CIRCULATING ANGIOGENIC FACTORS ELEVATED LIVER-ENZYMES REAL-TIME PCR NORMAL-PREGNANCY OXIDATIVE STRESS MATERNAL PLASMA FACTOR RECEPTOR-1 |
| Tema ODS | 03 Good Health and Well-being 05 Gender Equality |
| Tema ODS español | 03 Salud y bienestar 05 Igualdad de género |
| Tipo de documento | artículo |