Background & aims: Bile acid (BA) pool size remains unchanged after cholecystectomy (XGB) but it circulates faster, exposing the enterohepatic system to an increased flux of BA. Triglyceride (TG) and BA metabolisms are functionally inter-related. We investigated whether ablation of the gallbladder (GB) modifies hepatic TG metabolism. Methods: Male mice were subjected to XGB and fed a normal diet. In some experiments, mice received a 1% nicotinic acid diet to block lipolysis. Parameters of BA and TG metabolism, and microsomal triglyceride transfer protein (MTTP) activity were measured 1-2 months after XGB. Serum parameters, hepatic lipids and mRNA expression of genes of lipid metabolism were determined. Results: BA pool size and synthesis were normal, but biliary BA secretion doubled during the diurnal light phase in XGB mice. Serum and hepatic TG concentrations increased 25% (P < 0.02), and hepatic very-low-density lipoproteins (VLDL)-TG and apoB-48 productions increased 15% (P < 0.03) and 50% (P < 0.01), respectively, after XGB. Feeding a 1% nicotinic acid did normalize VLDL production. MTTP activity increased 15% (P < 0.005) after XGB. Hepatic free fatty acid (FFA) synthesis and content, and mRNA levels of lipid metabolism-related genes remained normal in XGD mice. Conclusions: XGB increased serum and hepatic TG levels, and VLDL production, which were restored to normal by nicotinic acid. The results suggest that FFA flux from adipose tissue to the liver is increased in XGB mice. They support the hypothesis that the GB has a role in the regulation of hepatic TG metabolism and that XGB may favour the accumulation of fat in the liver.
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Título | Cholecystectomy increases hepatic triglyceride content and very-low-density lipoproteins production in mice |
Autor | Amigo, Ludwig Husche, Constanze Zanlungo, Silvana Luetjohann, Dieter Arrese, Marco Miquel, Juan F. Rigotti, Attilio Nervi, Flavio |
Enlace | |
Revista | LIVER INTERNATIONAL |
Volumen | 31 |
Número de publicación | 1 |
Fecha de publicación | 2011 |
Tipo de documento | artículo |
Agencia financiadora | Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) |
DOI | 10.1111/j.1478-3231.2010.02361.x |
Derechos | acceso restringido |
Editorial | WILEY |
ISSN | 1478-3223 |
ISSN electrónico | 1478-3231 |
Id de publicación en Pubmed | MEDLINE:21040411 |
Id de publicación en WoS | WOS:000285011000007 |
Paginación | 13 páginas |
Palabra clave | cholecystectomy gallbladder hepatic fatty acid and triglyceride metabolism VLDL production BILIARY LIPID SECRETION FIBROBLAST-GROWTH-FACTOR BILE-ACIDS METABOLIC SYNDROME TRANSFER PROTEIN APOLIPOPROTEIN-B ENTEROHEPATIC CIRCULATION RAT HEPATOCYTES TRANSGENIC MICE FATTY LIVER |
Página final | 64 |
Página inicio | 52 |
Resumen | Background & aims: Bile acid (BA) pool size remains unchanged after cholecystectomy (XGB) but it circulates faster, exposing the enterohepatic system to an increased flux of BA. Triglyceride (TG) and BA metabolisms are functionally inter-related. We investigated whether ablation of the gallbladder (GB) modifies hepatic TG metabolism. Methods: Male mice were subjected to XGB and fed a normal diet. In some experiments, mice received a 1% nicotinic acid diet to block lipolysis. Parameters of BA and TG metabolism, and microsomal triglyceride transfer protein (MTTP) activity were measured 1-2 months after XGB. Serum parameters, hepatic lipids and mRNA expression of genes of lipid metabolism were determined. Results: BA pool size and synthesis were normal, but biliary BA secretion doubled during the diurnal light phase in XGB mice. Serum and hepatic TG concentrations increased 25% (P < 0.02), and hepatic very-low-density lipoproteins (VLDL)-TG and apoB-48 productions increased 15% (P < 0.03) and 50% (P < 0.01), respectively, after XGB. Feeding a 1% nicotinic acid did normalize VLDL production. MTTP activity increased 15% (P < 0.005) after XGB. Hepatic free fatty acid (FFA) synthesis and content, and mRNA levels of lipid metabolism-related genes remained normal in XGD mice. Conclusions: XGB increased serum and hepatic TG levels, and VLDL production, which were restored to normal by nicotinic acid. The results suggest that FFA flux from adipose tissue to the liver is increased in XGB mice. They support the hypothesis that the GB has a role in the regulation of hepatic TG metabolism and that XGB may favour the accumulation of fat in the liver. |
dc.subject.ods | 03 Good Health and Well-being |
dc.subject.odspa | 03 Salud y bienestar |