Development of a Hibernation-Inspired Preservation Strategy to Enhance the Clinical Translation of Photosynthetic Biomaterials

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dc.catalogadordfo
dc.contributor.authorCorrales-Orovio, Rocio
dc.contributor.authorCastillo, Valentina
dc.contributor.authorRozas Rojas, Pablo
dc.contributor.authorSchenck, Thilo L.
dc.contributor.authorEgaña, José Tomas
dc.date.accessioned2024-01-29T18:25:28Z
dc.date.available2024-01-29T18:25:28Z
dc.date.issued2023
dc.description.abstractPhotosynthetic biomaterials have emerged as a promising approach for delivering oxygen and other bioactive molecules in several biomedical applications. This technology is based on the use of standard biomaterials loaded with photosynthetic cells for the controlled release of oxygen at the target site. However, as well as for other cell-based approaches, a main drawback for their clinical translation is the low shelf-life of living materials. Here, the potential of inducing a dormant hibernation-inspired state to preserve photosynthetic biomaterials for clinical applications is explored. First, a protocol to preserve microalgae Chlamydomonas reinhardtii is optimized and then applied to photosynthetic scaffolds, showing that the viability and functionality of the biomaterial is preserved for up to 6 weeks. To evaluate the clinical viability of this approach, both fresh and preserved photosynthetic scaffolds are implanted in a full-skin defect mouse model. The safety of this approach is evaluated and confirmed by several means, including clinical parameters, histological assays, and local and systemic molecular analysis. Altogether, for the first time the successful preservation of photosynthetic biomaterials through a hibernation-inspired strategy is described here, which could have a tremendous impact for the clinical translation of these materials as well as other photosynthetic therapies., Photosynthetic biomaterials have garnered attention for local and controlled delivery of oxygen to tissues. A novel hibernation-inspired preservation method is presented, extending the lifespan of photosynthetic scaffolds. The research demonstrates successful preservation for up to 6 weeks without cryopreservation agents, demonstrating in vivo safety and applicability. This advancement significantly enhances the clinical potential of photosynthetic materials and related therapies.image
dc.fuente.origenWOS
dc.identifier.doi10.1002/adtp.202300299
dc.identifier.eissn2366-3987
dc.identifier.urihttps://doi.org/10.1002/adtp.202300299
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81037
dc.identifier.wosidWOS:001091045800001
dc.information.autorucEscuela de Ingeniería; Rozas Rojas, Pablo; S/I; 1270391
dc.information.autorucEscuela de Ingeniería; Castillo Salgado Valentina Fernanda; S/I; 1080975
dc.information.autorucEscuela de Ingeniería; Egaña Erazo Jose Tomas; 0000-0003-2624-6992; 1018715
dc.issue.numero12
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final12
dc.pagina.inicio1
dc.revistaAdvanced therapeutics
dc.rightsacceso restringido
dc.subjectBiomaterial preservation
dc.subjectMicroalgae
dc.subjectOxygen therapy
dc.subjectPhotosynthetic biomaterials
dc.subject.ddc600
dc.subject.deweyTecnologíaes_ES
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleDevelopment of a Hibernation-Inspired Preservation Strategy to Enhance the Clinical Translation of Photosynthetic Biomaterials
dc.typeartículo
dc.volumen6
dc.volumen6
sipa.codpersvinculados1270391
sipa.codpersvinculados1080975
sipa.codpersvinculados1018715
sipa.trazabilidadWOS;2023-11-11
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